铜绿假单胞菌 KNU-2B 从蓖麻油酸生产 7,10,12-三羟基-8(E)-十八烯酸。
Production of 7,10,12-trihydroxy-8(E)-octadecenoic acid from ricinoleic acid by Pseudomonas aeruginosa KNU-2B.
机构信息
School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Korea.
Department of Public Health Sciences, Graduate School, Korea University, Seoul, Korea.
出版信息
Biotechnol Lett. 2020 Aug;42(8):1547-1558. doi: 10.1007/s10529-020-02883-4. Epub 2020 Apr 3.
Microbial production of hydroxy fatty acids (HFAs) was widely studied because of important biological properties of HFAs. Among microorganisms producing HFAs, Pseudomonas aeruginosa PR3 was well known to produce various HFAs from different unsaturated fatty acids. Recently, a new variant species of P. aeruginosa PR3 was isolated and characterized, showing improved efficiency for producing 7,10-dihydroxy-8(E)-octadecenoic acid from oleic acid. In this study, we report the production of 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD) from ricinoleic acid by the newly isolated P. aeruginosa KNU-2B. TOD was efficiently produced from ricinoleic acid by KNU-2B with the maximum conversion yield of 56.7% under the optimum reaction conditions of pH 8.0 and 48-h incubation at 27 °C, 150 rpm. Under optimized reaction conditions, maximum TOD production reached 340.3 mg/100 mL of the culture. However, requirement of nutritional factors by KNU-2B for production of TOD were considerably different from those by PR3 strain.
微生物生产羟基脂肪酸（HFAs）因其具有重要的生物学特性而受到广泛研究。在生产 HFAs 的微生物中，铜绿假单胞菌 PR3 以能够从不同的不饱和脂肪酸生产各种 HFAs 而闻名。最近，分离并鉴定了铜绿假单胞菌 PR3 的一个新变种，该变种从油酸生产 7,10-二羟基-8(E)-十八烯酸的效率得到了提高。在这项研究中，我们报告了新分离的铜绿假单胞菌 KNU-2B 从蓖麻油酸生产 7,10,12-三羟基-8(E)-十八烯酸（TOD）的情况。在最适反应条件（pH 8.0，27°C 下 150rpm 孵育 48 小时）下，KNU-2B 能够有效地将蓖麻油酸转化为 TOD，转化率最高可达 56.7%。在优化的反应条件下，TOD 的最大产量达到 340.3mg/100mL 培养物。然而，KNU-2B 生产 TOD 所需的营养因素与 PR3 菌株的要求有很大的不同。
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