嗜温鞘氨醇杆菌和蜡样芽孢杆菌特定菌株对堆肥中油酸的转化作用

Oleic acid transformations by selected strains of Sphingobacterium thalpophilum and Bacillus cereus from composted manure.

作者信息

Kaneshiro T, Nakamura L K, Bagby M O

机构信息

National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA.

出版信息

Curr Microbiol. 1995 Jul;31(1):62-7. doi: 10.1007/BF00294636.

DOI:10.1007/BF00294636

PMID:7767231

Abstract

In a survey of 186 randomly selected microbial strains isolated from composted manure, 63 transformed oleic acid into three types of products: hydroxy fatty acid, fatty amide, and less polar oleyl lipid. Selection of oleic acid-transforming microorganisms was enhanced in nutrient agar supplemented with 0.1% (vol/vol) oleic acid at pH 7.2. Most of the 63 diverse isolates elicited inconsistent and poorly reproduced transformations. However, strains 142b (NRRL B-14797) transformed oleic acid to 10-hydroxystearic acid consistently, and strain 229b (NRRL B-14812) produced an octadecenamide. Taxonomic studies indicated that NRRL strain B-14797, possessing 1,3-dihydroxy-2-amino-15-methylhexadecane and sphinganine bases, was closely related to Sphingobacterium thalpophilum, and NRRL B-14812 was identified as Bacillus cereus.

摘要

在一项对从堆肥粪便中随机分离出的186种微生物菌株的调查中，63种菌株将油酸转化为三种类型的产物：羟基脂肪酸、脂肪酰胺和极性较小的油酰脂质。在pH 7.2、添加0.1%（体积/体积）油酸的营养琼脂中，油酸转化微生物的筛选得到了增强。63种不同的分离菌株中，大多数引发的转化不一致且难以重复。然而，菌株142b（NRRL B-14797）能持续地将油酸转化为10-羟基硬脂酸，菌株229b（NRRL B-14812）则产生一种十八碳烯酰胺。分类学研究表明，含有1,3-二羟基-2-氨基-15-甲基十六烷和鞘氨醇碱基的NRRL菌株B-14797与嗜盐碱鞘氨醇杆菌密切相关，而NRRL B-14812被鉴定为蜡样芽孢杆菌。