Harper C L, Boulton M E, Marcyniuk B, Tullo A B, Ridgway A E
University Department of Ophthalmology, Manchester, UK.
Eye (Lond). 1998;12 ( Pt 5):834-8. doi: 10.1038/eye.1998.214.
To assess endothelial integrity following corneal transplantation using human corneas stored in organ culture in the Manchester Eye Bank.
A prospective study was undertaken on 24 patients who had received full-thickness corneal grafts using corneas stored in organ culture. The donor corneal endothelium was photographed prior to transplantation using light microscopy. Specular microscopy and ultrasonic pachometry were performed at 30 days (+/- 3 days), 12 weeks (+/- 1 week), 26 weeks (+/- 2 weeks) and 52 weeks (+/- 4 weeks) following corneal transplantation. The following cell parameters were measured: density, area, coefficient of variation (CV) for area, perimeter, diameter, form factor and corneal thickness.
One year after corneal transplantation there was a statistically significant decrease in endothelial cell density (-39.4%), and a statistically significant increase in endothelial cell area (+94.4%), perimeter (+36.1%), diameter (+57.1%) and form factor (+5.8%). However, no significant changes were seen in CV or corneal thickness with respect to time after transplantation. (There were no significant changes in endothelial cell parameters and corneal thickness for 12 control subjects.)
Endothelial cell loss occurs at an accelerated rate from corneal transplants. This highlights the need for improving corneal endothelial viability during and after storage in order to improve the chances of longer-term survival of the transplanted cornea.
使用曼彻斯特眼库器官培养保存的人角膜评估角膜移植后的内皮完整性。
对24例接受使用器官培养保存角膜的全层角膜移植患者进行前瞻性研究。移植前使用光学显微镜对供体角膜内皮进行拍照。在角膜移植后30天（±3天）、12周（±1周）、26周（±2周）和52周（±4周）进行镜面显微镜检查和超声测厚。测量以下细胞参数：密度、面积、面积变异系数（CV）、周长、直径、形态因子和角膜厚度。
角膜移植一年后，内皮细胞密度有统计学意义的下降（-39.4%），内皮细胞面积、周长、直径和形态因子有统计学意义的增加（分别为+94.4%、+36.1%、+57.1%和+5.8%）。然而，移植后不同时间的CV或角膜厚度未见显著变化。（12名对照受试者的内皮细胞参数和角膜厚度无显著变化。）
角膜移植后内皮细胞损失加速。这凸显了在储存期间和之后提高角膜内皮活力的必要性，以提高移植角膜长期存活的机会。
