Bøgh H O, Zhu X Q, Qian B Z, Gasser R B
Department of Veterinary Science, University of Melbourne, Victoria 3030, Australia.
Parasitology. 1999 Jan;118 ( Pt 1):73-82. doi: 10.1017/s0031182098003527.
In this study, we employed a mutation scanning approach for the direct visual display of genetic variability in mitochondrial DNA (mtDNA) fragments within and among populations of Schistosoma japonicum from the People's Republic of China. Fragments of the NADH dehydrogenase 1 gene (ND1) and the cytochrome c oxidase subunit I (COI) were individually amplified from parasite DNA by polymerase chain reaction (PCR), denatured and subjected to single-strand conformation polymorphism (SSCP) analysis. Using ND1 and COI fragments, individuals representing different genotypes could be readily identified based on characteristic and reproducible SSCP profiles. The results demonstrated the usefulness of SSCP for the direct visual display of low-level sequence variation in mtDNA of S. japonicum prior to DNA sequence analysis. This approach has important implications for studying the genetic structure and biology of S. japonicum populations, and for analysing the inheritance of mitochondrial DNA.
在本研究中，我们采用了一种突变扫描方法，用于直接直观地展示中华人民共和国日本血吸虫种群内部和种群之间线粒体DNA（mtDNA）片段的遗传变异性。通过聚合酶链反应（PCR）从寄生虫DNA中分别扩增烟酰胺腺嘌呤二核苷酸脱氢酶1基因（ND1）片段和细胞色素c氧化酶亚基I（COI）片段，将其变性后进行单链构象多态性（SSCP）分析。利用ND1和COI片段，根据特征性且可重复的SSCP图谱能够轻松鉴定出代表不同基因型的个体。结果表明，在进行DNA序列分析之前，SSCP对于直接直观地展示日本血吸虫mtDNA中的低水平序列变异是有用的。该方法对于研究日本血吸虫种群的遗传结构和生物学特性，以及分析线粒体DNA的遗传具有重要意义。
