Liu Wei, Liu Yi, Lin Rui-Qing, Dai Rong-Si, Cheng Tian-Yin, Zhu Xing-Quan
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2008 Apr;26(2):152-3.
The partial NADH dehydrogenase subunit 4 (pnad4) fragment was amplified by polymerase chain reaction (PCR) from Schistosoma japonicum collected from 4 areas in Hunan province, and the amplicons were cloned and sequenced. A 480 bp sequence was obtained and the nucleotide difference between samples from the four locations were 0.4%-1.3%. Low level of sequence variability between and within different populations of S. japonicum was detected. The results are worthwhile for further study on the population genetic structures of S. japonicum.
通过聚合酶链反应（PCR）从采自湖南省4个地区的日本血吸虫中扩增出部分烟酰胺腺嘌呤二核苷酸脱氢酶亚基4（pnad4）片段，对扩增产物进行克隆和测序。获得了一段480 bp的序列，4个地点样本之间的核苷酸差异为0.4%-1.3%。检测到日本血吸虫不同群体之间和群体内部的序列变异水平较低。该结果对于进一步研究日本血吸虫的群体遗传结构具有重要价值。
