Kakinuma C, Suda K, Shibutani Y
Department of Pathology, Juntendo University, School of Medicine, Tokyo, Japan.
Virchows Arch. 1999 Jan;434(1):83-9. doi: 10.1007/s004280050309.
We investigated the time-course of changes in pancreatic fibrosis accompanied with pancreatitis in WBN/Kob rats. The areas of fibrosis and fatty replacement were analysed morphometrically, and biochemical measurements of pancreatic and plasma prolyl hydroxylase and of pancreatic collagenase were assessed. Male rats showed acute pancreatitis at 2-3 months of age, lesions that later underwent a transition to widespread fibrosis. The fibrosis then decreased, and the fibrotic tissue was replaced with adipose tissue. Morphometrically, the fibrotic area reached its maximal size when the rats were 4 months old, diminishing thereafter. The fibrosis occurred mainly in the intralobular space, and was principally attributable to type-III collagen. Type-I collagen scarcely appeared throughout the experimental period. Alpha-Smooth muscle actin appeared in and around myofibroblasts that developed in an early stage and diminished later in accordance with the progressive manner of fibrosis. The plasma prolyl hydroxylase level was higher in males than in females from 4 through 10 months of age. Pancreatic collagenase activity in the males also increased during the same period. These findings suggest that pancreatic fibrosis in male WBN/Kob rats is affected by the balance between prolyl hydroxylase and collagenase.
我们研究了WBN/Kob大鼠胰腺炎伴发的胰腺纤维化的时间进程变化。对纤维化和脂肪替代区域进行形态计量分析，并评估胰腺和血浆脯氨酰羟化酶以及胰腺胶原酶的生化指标。雄性大鼠在2至3月龄时出现急性胰腺炎，随后病变转变为广泛纤维化。之后纤维化程度降低，纤维化组织被脂肪组织替代。形态计量学结果显示，大鼠4月龄时纤维化面积达到最大，此后逐渐减小。纤维化主要发生在小叶内间隙，主要归因于III型胶原。在整个实验期间，I型胶原几乎未出现。α-平滑肌肌动蛋白出现在早期形成的肌成纤维细胞及其周围，随后随着纤维化进程逐渐减少。4至10月龄雄性大鼠的血浆脯氨酰羟化酶水平高于雌性。同一时期，雄性大鼠的胰腺胶原酶活性也有所增加。这些发现表明，雄性WBN/Kob大鼠的胰腺纤维化受脯氨酰羟化酶和胶原酶之间平衡的影响。
