Mori Y, Yokoyama J, Nishimura M, Kurata H, Miura J, Ikeda Y
Third Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
Pancreas. 1990 Jul;5(4):452-9. doi: 10.1097/00006676-199007000-00013.
A spontaneously developed endocrine-exocrine pancreatic dysfunction was observed in the aged males of an inbred strain of Wistar rats, WBN/Kob. Nonobese male WBN/Kob rats developed glycosuria and hyperglycemia at around 9 months of age. Cumulative incidence of diabetes in male rats was 43% (33 of 76) at 12 months of age and reached 90% at the age of 21 months. In contrast, female rats did not become diabetic. Urinary excretion of amylase in WBN/Kob rats was significantly increased in comparison with control Wistar rats. Moreover, the exocrine pancreatic function test was impaired in WBN/Kob rats. Pathological examination of pancreata revealed infiltration of inflammatory cells, hemorrhage, deposition of hemosiderin, and fibrinous exudation around pancreatic ducts and blood vessels at 3 months of age. A gradual increase of fibrous tissue into the exocrine tissue and islets was observed with advancing age. The extremely enlarged interlobular lymph nodes were also observed. At the age of 12 months, the fibrous tissue replaced extensive areas of the pancreas and involved islets. The amylase content of pancreata in WBN/Kob rats was markedly decreased in comparison with that in Wistar rats at 12 months of age. Islets composed of few endocrine cells were detected. Immunohistochemical staining for insulin and glucagon showed a decreased number of not only B cells but also A cells. Moreover, both the pancreatic insulin and glucagon contents were markedly decreased in WBN/Kob rats in comparison with Wistar rats.(ABSTRACT TRUNCATED AT 250 WORDS)
在近交系Wistar大鼠WBN/Kob的老年雄性大鼠中观察到一种自发产生的内分泌-外分泌胰腺功能障碍。非肥胖雄性WBN/Kob大鼠在9个月龄左右出现糖尿和高血糖。雄性大鼠在12个月龄时糖尿病的累积发病率为43%（76只中有33只），在21个月龄时达到90%。相比之下，雌性大鼠未患糖尿病。与对照Wistar大鼠相比，WBN/Kob大鼠的淀粉酶尿排泄量显著增加。此外，WBN/Kob大鼠的外分泌胰腺功能测试受损。胰腺的病理检查显示，在3个月龄时，胰腺导管和血管周围有炎性细胞浸润、出血、含铁血黄素沉积和纤维蛋白渗出。随着年龄的增长，观察到纤维组织逐渐侵入外分泌组织和胰岛。还观察到小叶间淋巴结极度肿大。在12个月龄时，纤维组织取代了胰腺的大片区域并累及胰岛。与12个月龄的Wistar大鼠相比，WBN/Kob大鼠胰腺中的淀粉酶含量显著降低。检测到由少量内分泌细胞组成的胰岛。胰岛素和胰高血糖素的免疫组织化学染色显示，不仅B细胞数量减少，A细胞数量也减少。此外，与Wistar大鼠相比，WBN/Kob大鼠胰腺中的胰岛素和胰高血糖素含量均显著降低。（摘要截断于250字）
