[某些分析方法低估血液一氧化碳的风险]

[Risk of underestimating blood carbon monoxide by certain analytic methods].

作者信息

Gourlain H, Laforge M, Buneaux F, Galliot-Guilley M

机构信息

Laboratoire de Biochime-Toxicologie, Hôpital Fernand Widal, Paris.

出版信息

Presse Med. 1999 Jan 30;28(4):163-7.

PMID:10071626

Abstract

OBJECTIVE

Study the effect of delay to assay on the measurement of carboxyhemoglobin (HbCO) in total blood samples.

METHODS

Carbon monoxide (CO) and carboxyhemoglobin were measured on 75 blood samples drawn from healthy subjects (smokers and non smokers) and in subjects with carbon monoxide (CO) poisoning. Blood samples were drawn on lithium heparinate in perfectly closed tubes with no head space and stored at 4 infinity C until assay. The samples were pooled into 4 classes for 4 delays to assay: immediate, less than one hour, 3 hours, 12 hours. Infrared spectrometry was used to assay CO and order 4 and 5 derived spectrophotometry using CO-oximeters (AVL 912, IL 482, Corning 270, Radiometer OSM 3, Radiometer ABL 520) for HbCO.

RESULTS

Regression lines for CO versus HbCO suggested that oxycarbonemia was underestimated using techniques measuring HbCO. This underestimation varied from 3 to 40% for delays to assay of 0 to 3 hours.

CONCLUSION

Clinicians should be aware that the underestimation in oxycarbonemia related to HbCO assays is sensitive to delay to assay.

摘要

目的

研究检测延迟对全血样本中碳氧血红蛋白（HbCO）测量的影响。

方法

对从健康受试者（吸烟者和非吸烟者）以及一氧化碳（CO）中毒受试者采集的75份血样进行一氧化碳（CO）和碳氧血红蛋白检测。血样采集于装有肝素锂的完全封闭、无顶空的试管中，保存在4℃直至检测。将样本分为4组，分别对应4种检测延迟时间：即时检测、小于1小时、3小时、12小时。采用红外光谱法检测CO，使用CO血氧计（AVL 912、IL 482、康宁270、Radiometer OSM 3、Radiometer ABL 520）通过衍生分光光度法检测HbCO。