一种用于一氧化碳（CO）暴露诊断的新方法：测量人体血液中的总一氧化碳与碳氧血红蛋白（HbCO）含量。
A new approach for the carbon monoxide (CO) exposure diagnosis: measurement of total CO in human blood versus carboxyhemoglobin (HbCO).
作者信息
Varlet Vincent, De Croutte Emma Lagroy, Augsburger Marc, Mangin Patrice
机构信息
University Center of Legal Medicine Lausanne - Geneva, Forensic Toxicology and Chemistry Unit, CH-1011, Lausanne, Switzerland.
出版信息
J Forensic Sci. 2013 Jul;58(4):1041-6. doi: 10.1111/1556-4029.12130. Epub 2013 May 21.
The aim of the study is to present the application of a headspace-gas chromatography-mass spectrometry (HS-GC-MS) method for the determination of the carbon monoxide (CO) blood concentration and to compare it with carboxyhemoglobin (HbCO) saturation. In postmortem cases, the HbCO measured by spectrophotometry frequently leads to inaccurate results due to inadequate samples or analyses. The true role of CO intoxication in the death of a person could be misclassified. The estimation of HbCO from HS-GC-MS CO measurements provides helpful information by determining the total CO levels (CO linked to hemoglobin (HbCO) and CO dissociated from hemoglobin). The CO concentrations were converted in HbCO saturation levels to define cutoff blood CO values. CO limits were defined as less than 1 μmol/mL for living persons, less than 1.5 μmol/mL for dead persons without CO exposure, and greater than 3 μmol/mL for dead persons with clear CO poisoning.
本研究的目的是介绍一种顶空气相色谱-质谱联用（HS-GC-MS）方法在测定血液中一氧化碳（CO）浓度方面的应用，并将其与碳氧血红蛋白（HbCO）饱和度进行比较。在尸检案例中，由于样本或分析不充分，通过分光光度法测量的HbCO常常导致结果不准确。CO中毒在人员死亡中所起的真正作用可能会被错误分类。通过HS-GC-MS测量CO来估算HbCO，通过测定总CO水平（与血红蛋白结合的CO（HbCO）和从血红蛋白解离的CO）可提供有用信息。将CO浓度转换为HbCO饱和度水平，以确定血液CO的临界值。CO限值定义为：活人小于1μmol/mL，无CO暴露的死者小于1.5μmol/mL，有明确CO中毒的死者大于3μmol/mL。
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