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[斯维尔德洛夫斯克病毒感染研究所馆藏连续传代细胞的葡萄糖-6-磷酸脱氢酶和乳酸脱氢酶同工酶特性]

[Isoenzyme characteristics of glucose-6-phosphage dehydrogenase and lactate dehydrogenase of continuous cells from the collection of the Sverdlovsk Research Institute of Virus Infections].

作者信息

Tsareva A A, Glinskikh N P, Dreĭzin R S, Kolesnikova G G, Zhdanov V M

出版信息

Vopr Virusol. 1976 Sep-Oct(5):620-3.

PMID:1007232
Abstract

An analysis of isoenzymes of glucose-6-phosphage dehydrogenase (G-6-P-DH) and lactage dehydrogenase (LDH) of continuous cells from the collection of Sverdlovsk Research Institute of Virus Infections was carried out. Seventeen continous human and animal cell lines were examined by electrophoresis in a vertical block of 7% polyacrylamide gel followed by histochemical detection of the enzymes. The HEp-2, HEp-2 clone No. 23, KB, RH, and FL cells were shown to have the electrophoretic motility of G-6-PDG characteristic of HeLa cell line. LEP and SOC cell lines were contaminated with mouse cells. A culture of rat fibroblasts had isoenzymes of G-6-PDG and LDG characteristic of HeLa cell line. The remaining cell lines had the isoenzymatic characteristics corresponding to their species appurtenance.

摘要

对斯维尔德洛夫斯克病毒感染研究所保藏的连续传代细胞的葡萄糖-6-磷酸脱氢酶(G-6-P-DH)和乳酸脱氢酶(LDH)同工酶进行了分析。通过在7%聚丙烯酰胺凝胶垂直板上进行电泳,随后对酶进行组织化学检测,对17种连续传代的人和动物细胞系进行了检查。结果显示,HEp-2、HEp-2第23号克隆、KB、RH和FL细胞具有HeLa细胞系特有的G-6-PDG电泳迁移率。LEP和SOC细胞系被小鼠细胞污染。大鼠成纤维细胞培养物具有HeLa细胞系特有的G-6-PDG和LDG同工酶。其余细胞系具有与其物种归属相对应的同工酶特征。

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