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[昆虫细胞系的鉴定]

[Identification of insect cell lines].

作者信息

Tsareva A A, Kolokol'tsova T D, Nemtsov Iu V, Isaenko A A, Bochkova T G

出版信息

Vopr Virusol. 1987 Jan-Feb;32(1):87-95.

PMID:3554756
Abstract

Identification of 10 cell lines of 6 insect species received by the cell culture collection of Molecular Biology Institute from different research institutions of the USSR was carried out by karyological analysis and determinations of the spectrum of isoforms of the following enzymes: glucose-6-phosphate dehydrogenase (G-6-PDH), lactate dehydrogenase (LDH), isocitrate dehydrogenase (ICDH), and superoxide dismutase (SOD). Nine out of the 10 cell lines examined were shown to be identical to those of gypsy moth. Possible ways of cell contamination with other cells in long-term cultivation and preparation of new lines were determined.

摘要

对苏联不同研究机构提供给分子生物学研究所细胞培养库的6种昆虫的10个细胞系进行了核型分析,并测定了以下几种酶的同工型谱:葡萄糖-6-磷酸脱氢酶(G-6-PDH)、乳酸脱氢酶(LDH)、异柠檬酸脱氢酶(ICDH)和超氧化物歧化酶(SOD)。结果表明,所检测的10个细胞系中有9个与舞毒蛾的细胞系相同。确定了长期培养和新细胞系制备过程中细胞被其他细胞污染的可能途径。

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