[体外循环对肺表面活性物质活性的影响]

[The effects of cardiopulmonary bypass on pulmonary surfactant activity].

作者信息

Xing Q, Zhang S, Chen Z

机构信息

Department of Cardiothoracic Surgery, Children's Hospital, Shanghai Medical University.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 1997 Aug;20(4):225-7.

PMID:10072808

Abstract

OBJECTIVE

To study the effects of cardiopulmonary by pass(CPB) on pulmonary surfactant (PS) activity.

METHOD

In 12 children with patent ductus arteriosus(PDA) and 19 patients with ventricular septal defect (VSD), saturated phosphatidylcholine (SatPC), total phospholipids (TPL), total protein (TP) and surfactant protein-A(SP-A) in sequential airway aspirates before and after operation were determined. Tetroxide osmium digestion and neutral alumina column chromatography were used for SatPC, and a modified immunoblot method with a rabbit anti-human SP-A polyclonal antibody for SP-A measurment.

RESULTS

In 19 VSD patients with CPB, SatPC/TPL was significantly decreased from 48% to 34% (t = 2.737, P < 0.05), SatPC/TP decreased from 64 to 33 mg/g (t = 3.011, P < 0.01), and SP-A from 23 to 11mg/g (t = 2.987, P < 0.01). In 12 patients without CPB, SatPC/TPL, SatPC/TP, SP-A/TP levels were not changed.

CONCLUSIONS

CPB significantly reduces pulmonary surfactant activity.

摘要

目的

研究体外循环（CPB）对肺表面活性物质（PS）活性的影响。

方法

对12例动脉导管未闭（PDA）患儿和19例室间隔缺损（VSD）患者，测定手术前后序贯气道吸出物中的饱和磷脂酰胆碱（SatPC）、总磷脂（TPL）、总蛋白（TP）和表面活性蛋白-A（SP-A）。SatPC采用四氧化锇消化法和中性氧化铝柱色谱法测定，SP-A采用兔抗人SP-A多克隆抗体的改良免疫印迹法测定。

结果

19例接受CPB的VSD患者中，SatPC/TPL从48%显著降至34%（t = 2.737，P < 0.05），SatPC/TP从64降至33 mg/g（t = 3.011，P < 0.01），SP-A从23降至11mg/g（t = 2.987，P < 0.01）。12例未接受CPB患者的SatPC/TPL、SatPC/TP、SP-A/TP水平未发生变化。