Gan H, Ouyang Q, Bu H
Department of Internal Medicine, First Affiliated Hospital, West China University of Medical Sciences, Chengdu.
Zhonghua Jie He He Hu Xi Za Zhi. 1997 Jun;20(3):149-52.
To evaluate the diagnostic value of polymerase chain reaction (PCR) assay in combination with Southern hybridization technique and enzyme-linked immunosorbent assay (ELISA) in tuberculous peritonitis.
Fourty-two tuberculous ascites were detected for M. tuberculosis by PCR technique in combination with Southern hybridization, in which nuclear acid probe was digoxigenin-labeled, and the result was compared with conventional bacteriological methods and ELISA. IS6110, a specific insert sequence of M. tuberculosis, was used as primer. The specificity of PCR products was confirmed by hybridization and digestion with Sal I restrictive endonuclerase, and the sensitivity of hybridization was compared with gel electrophoresis.
The sensitivities were 69% by PCR, 71% by ELISA, 9% by culture and 0 by acid fast staining respectively. Hybridization was found more sensitive than gel electrophoresis.
Both PCR technique and ELISA are valuable in diagnosis of tuberculous peritonitis, and the former is more specific than the latter. PCR technique in combination with Southern hybridization can improve both the sensitivity and the specificity of dectection.
评估聚合酶链反应（PCR）检测联合Southern杂交技术及酶联免疫吸附测定（ELISA）在结核性腹膜炎诊断中的价值。
采用PCR技术联合Southern杂交检测42例结核性腹水标本中的结核分枝杆菌，核酸探针采用地高辛标记，结果与传统细菌学方法及ELISA进行比较。以结核分枝杆菌特异性插入序列IS6110为引物。PCR产物的特异性通过与Sal I限制性内切酶杂交及酶切进行确认，杂交敏感性与凝胶电泳进行比较。
PCR、ELISA、培养及抗酸染色的敏感性分别为69%、71%、9%及0。发现杂交比凝胶电泳更敏感。
PCR技术和ELISA在结核性腹膜炎诊断中均有价值，前者比后者更具特异性。PCR技术联合Southern杂交可提高检测的敏感性和特异性。
