Gan H, Ouyang Q, Bu H, Li S, Chen D, Li G, Yang X
Department of Gastroenterology, West China University of Medical Sciences, Chengdu.
Chin Med J (Engl). 1995 Mar;108(3):215-20.
It is difficult to make a precise diagnosis of intestinal tuberculosis and to differentiate it from Crohn's disease. For evaluating Polymerase Chain Reaction (PCR) assay in these two aspects, 36 specimens of intestinal tuberculosis from surgical resections and endoscopic biopsies and 26 Crohn's disease samples were subjected to PCR assay. 21 specimens of normal colon tissue surrounding cancer were used as the control. Oligonucleotides derived from the IS 6110 sequence, which is repeated in M. tuberculosis chromosome and highly specific for the M. tuberculosis complex, were used as a primer. The amplified PCR products were detected by examination of ethidium-bromide-stained polyacrylamide gels. The specificity of PCR products was confirmed by digestion with Sal I restrictive endonuclease and southern blot hybridization using digoxigenin-labeled probe. The results showed that the M. tuberculosis DNA was identified in 27/36 intestinal tuberculosis, but none of 26 Crohn's disease. Acid fast bacilli were only found in 16/36 intestinal tuberculosis. In conclusion, as a rapid, sensitive, and specific pathogenic method in diagnosis of intestinal tuberculosis, PCR assay has been developed in this study, and is considered valuable in the differentiation between intestinal tuberculosis and Crohn's disease.
肠结核的准确诊断以及与克罗恩病的鉴别诊断存在困难。为了在这两个方面评估聚合酶链反应（PCR）检测，对36例手术切除和内镜活检获得的肠结核标本以及26例克罗恩病样本进行了PCR检测。将21例癌旁正常结肠组织标本用作对照。以来源于结核分枝杆菌染色体中重复出现且对结核分枝杆菌复合群具有高度特异性的IS 6110序列的寡核苷酸作为引物。通过检查溴化乙锭染色的聚丙烯酰胺凝胶来检测扩增的PCR产物。用Sal I限制性内切酶消化并用地高辛标记的探针进行Southern印迹杂交来确认PCR产物的特异性。结果显示，在36例肠结核中有27例检测到结核分枝杆菌DNA，而26例克罗恩病中均未检测到。仅在36例肠结核中的16例发现抗酸杆菌。总之，本研究开发了一种作为诊断肠结核的快速、灵敏且特异的病原学方法，PCR检测在肠结核与克罗恩病的鉴别诊断中具有重要价值。
