[氧氟沙星抗结核活性的实验研究]

[Experimental study on the antituberculous activity of ofloxacins].

作者信息

Duan L, Liang G, Li H

机构信息

Beijing Tuberculosis and Thoracic Tumor Research Institute.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 1997 Apr;20(2):98-100.

PMID:10072834

Abstract

OBJECTIVE

To compare the antituberculous activities of ofloxacins (OFLX) made by different pharmaceutical companies.

METHODS

The minimal inhibitory concentrations (MIC) of OFLX were determined by two-fold dilution method. The efficacy of OFLX was compared in terms of median survival time (ST50) against M. tuberculosis infection in mice.

RESULTS

In vitro the activities against M. tuberculosis (H37Rv), M. bovis (Ravenel) and M. Phlei products showed similar degree. The MICs of OFLX were 0.5-1.0 microgram/ml in modified Sauton medium and 1.0 microgram/ml in modified Lowenstin-Jensen medium. OFLX was more active than ethambutol (MIC 4-8 micrograms/ml, 2.0 micrograms/ml, respectively) against M. tuberculosis in the same media. OFLX from domestic or foreign drug companies showed similar results for treating M. tuberculosis infection in mice and all were significantly effective. The efficacy of OFLX at the dose of 4 mg per mouse was found close to that of ethambutol (EMB) at the dose of 2 mg per mouse. The efficacy of OFLX at the dose of 2 mg per mouse close to EMB at the dose of 1 mg per mouse and thiacetazone at the dose of 2 mg per mouse.

CONCLUSIONS

In vitro and in vivo, the activities of OFLX from different sources against M. tuberculosis are about the same.

摘要

目的

比较不同制药公司生产的氧氟沙星（OFLX）的抗结核活性。

方法

采用二倍稀释法测定OFLX的最低抑菌浓度（MIC）。根据小鼠感染结核分枝杆菌后的中位生存时间（ST50）比较OFLX的疗效。

结果

体外实验中，OFLX对结核分枝杆菌（H37Rv）、牛分枝杆菌（Ravenel）和草分枝杆菌的活性表现出相似程度。在改良苏通培养基中，OFLX的MIC为0.5 - 1.0微克/毫升，在改良罗氏培养基中为1.0微克/毫升。在相同培养基中，OFLX对结核分枝杆菌的活性比乙胺丁醇（MIC分别为4 - 8微克/毫升、2.0微克/毫升）更强。国内外制药公司生产的OFLX在治疗小鼠结核分枝杆菌感染方面结果相似，且均有显著疗效。发现每只小鼠剂量为4毫克的OFLX的疗效接近每只小鼠剂量为2毫克的乙胺丁醇（EMB）的疗效。每只小鼠剂量为2毫克的OFLX的疗效接近每只小鼠剂量为1毫克的EMB和每只小鼠剂量为2毫克的氨硫脲的疗效。