Fu J, Zheng J, Wu B
Department of Pathology, Beijing Medical University.
Zhonghua Bing Li Xue Za Zhi. 1997 Aug;26(4):214-7.
Interleukin-6 (IL-6) has previously been implicated as a potential positive autocrine regulator of a human lung carcinoma cell lince PG. The purpose of the present study is to further evaluate the role of IL-6 in PG cells in vivo.
An antisense expression vector for IL-6 was constructed and introduced into PG cells. Antisense mRNA of IL-6 was detected by RNA-RNA dot blotting analysis. The production of bioactive IL-6 was measured by bioassay method. To determine the effect of antisense IL-6 cDNA on tumorigenicity, PG cells were inoculated subcutaneously into nude mice.
Four transfectants, PGTAS1, PGTAS6, PGTAS8, PGTAS9, could express IL-6 antisense mRNA and secret decreased bioactive IL-6. The growth rate of 4 transfectants revealed was reduced in vitro. Comparing with the mice injected with the control PGTneo cells, the latent period of the mice inoculated with PGTAS6, PGTAS8, PGTAS9 cells was significantly increased, and the growth rate of the tumors was obviously decreased. The tumor size was markedly smaller. There was a negative correlation between the growth-inhibiting effect of IL-6 antisense gene and the amount of IL-6 secretion.
These results indicate that IL-6 can function as an autocrine stimulator for PG cells in vivo as well as in vitro.
白细胞介素-6（IL-6）先前被认为是一种人类肺癌细胞系PG潜在的正性自分泌调节因子。本研究的目的是进一步评估IL-6在PG细胞体内的作用。
构建IL-6的反义表达载体并导入PG细胞。通过RNA-RNA斑点杂交分析检测IL-6的反义mRNA。采用生物测定法测定生物活性IL-6的产生。为了确定反义IL-6 cDNA对致瘤性的影响，将PG细胞皮下接种到裸鼠体内。
四个转染子，PGTAS1、PGTAS6、PGTAS8、PGTAS9，可表达IL-6反义mRNA并分泌减少的生物活性IL-6。所检测的4个转染子在体外的生长速率降低。与注射对照PGTneo细胞的小鼠相比，接种PGTAS6、PGTAS8、PGTAS9细胞的小鼠潜伏期显著延长，肿瘤生长速率明显降低。肿瘤大小明显更小。IL-6反义基因的生长抑制作用与IL-6分泌量之间呈负相关。
这些结果表明，IL-6在体内和体外均可作为PG细胞的自分泌刺激因子。
