Bian X, Shi J, Liu F
Department of Pathology, Third Military Medical University, Chongqing.
Zhonghua Bing Li Xue Za Zhi. 1997 Apr;26(2):89-92.
To explore the relationship between the proliferative activities, oncoprotein expression, cell differentiation and prognosis of gliomas.
Immunohistochemistry and image analysis were used to study the expression of proliferating cell nuclear antigen (PCNA) and several oncoproteins both qualitatively and quantitatively in 124 brain gliomas.
It was found that the intensities of PCNA reaction were significantly related to both the grade and prognosis of gliomas. Overexpression of c-erbB-2 protein was slightly stronger in well than in poorly-differenciated gliomas. Moreover, the reactions in patients who survived over 5 years were stronger than in those under 5 years. EGF (40.0%), EGFR (91.4%) and p21ras (53.3%) expression levels were related to neigher the grading nor prognosis of this tumor. The positive ratios of the three antibodies to p53 protein were higher in grades II-IV than in grade I gliomas. The intensity of p53 reaction was correlated to that of PCNA.
It is suggested that the aberration of c-erbB-2, p21ras, EGF and EGFR might be the early events in the initiation and progression of gliomas, whereas p53 is involved in all stages of these tumors. PCNA could reflect the degree of malignancy to a certain extent.
探讨神经胶质瘤的增殖活性、癌蛋白表达、细胞分化与预后之间的关系。
采用免疫组织化学和图像分析技术，对124例脑胶质瘤中增殖细胞核抗原（PCNA）及几种癌蛋白的表达进行定性和定量研究。
发现PCNA反应强度与神经胶质瘤的分级及预后均显著相关。c-erbB-2蛋白在高分化神经胶质瘤中的过表达略强于低分化神经胶质瘤。此外，生存超过5年患者的反应强于生存不足5年的患者。EGF（40.0%）、EGFR（91.4%）和p21ras（53.3%）的表达水平与该肿瘤的分级和预后均无关。p53蛋白三种抗体的阳性率在Ⅱ-Ⅳ级神经胶质瘤中高于Ⅰ级神经胶质瘤。p53反应强度与PCNA反应强度相关。
提示c-erbB-2、p21ras、EGF和EGFR的异常可能是神经胶质瘤发生和发展的早期事件，而p53参与了这些肿瘤的各个阶段。PCNA在一定程度上可反映恶性程度。
