Bian X W, Shi J Q, Liu F X
Institute of Pathology, Southwestern Hospital, Third Military Medical University, Chongqing City, People's Republic of China.
Anal Quant Cytol Histol. 2000 Dec;22(6):429-37.
To clarify the clinicopathologic significance of immunohistochemistry for proliferative activity and oncoprotein expression in astrocytic tumors.
Ninety-seven cases of brain astrocytic tumors with histologic grading and follow-up data were investigated with immunohistochemistry and image analyzer to detect the expression of proliferating cell nuclear antigen (PCNA), silver-binding nucleolar organizer regions (AgNORs) and several oncoproteins.
PCNA was significantly related to AgNORs, grading and prognosis of astrocytomas. The frequency of mutant p53 protein expression was higher in grade 2-4 astrocytomas than in grade 1. Epidermal growth factor (EGF) (37.1%), EGF receptor (83.5%) and p21ras (42.3%) expression levels were related to neither the grade nor prognosis of the tumors. The positive ratios of p53 antibodies were higher in grades 2-4 than in grade 1, and the intensities correlated with PCNA but not with prognosis.
Aberrations of c-erbB-2, p21ras, EGF and EGF receptor might be early events in the initiation and progression of astrocytomas, whereas p53 overexpression is involved in all the stages. Immunohistochemical detection had no prognostic value. PCNA could be important to the evaluation of astrocytoma malignancy.
阐明免疫组织化学检测在星形细胞瘤增殖活性及癌蛋白表达方面的临床病理意义。
采用免疫组织化学及图像分析仪对97例有组织学分级及随访数据的脑星形细胞瘤进行研究，检测增殖细胞核抗原（PCNA）、银结合核仁组成区（AgNORs）及几种癌蛋白的表达。
PCNA与AgNORs、星形细胞瘤分级及预后显著相关。2-4级星形细胞瘤中突变型p53蛋白表达频率高于1级。表皮生长因子（EGF）（37.1%）、表皮生长因子受体（83.5%）及p21ras（42.3%）的表达水平与肿瘤分级及预后均无关。2-4级p53抗体阳性率高于1级，其强度与PCNA相关，但与预后无关。
c-erbB-2、p21ras、EGF及表皮生长因子受体的异常可能是星形细胞瘤发生及进展的早期事件，而p53过表达则涉及所有阶段。免疫组织化学检测无预后价值。PCNA对评估星形细胞瘤恶性程度可能具有重要意义。
