Zeng L, Cui W, Fang W
Department of Pathology, Beijing Medical University.
Zhonghua Bing Li Xue Za Zhi. 1997 Apr;26(2):93-5.
To study the difference in expression and response of certain growth factors between the two metastatic variants PGbE1 and PGLH7 of human pulmonary giant cell carcinoma, and the action of these growth factors on the metastasis of tumor cells.
RT-PCT was conducted to detect the expression of TGF alpha, TGF beta 1, IL-6, IL-8, bFGF and ANG, and the expression of receptors EGFR, IL-6R and IL-8R; 3H-TdR incorporation assay was used to determine the effects of recombinant TGF alpha, TGF beta 1 and IL-6 on the proliferation of the two cells.
TGF alpha, EGFR, IL-6 and IL-6R were expressed at a higher level in PGbE1 cells than in PGLH7 cells. No significant differences were found in the expression of TGF beta 1, bFGF, IL-8, IL-8R and ANG between the two cells. Recombinant TGF alpha and IL-6 stimulated the proliferation of both cells, while TGF beta 1 had dual effects.
TGF alpha, TGF beta 1, bFGF, IL-6, IL-8 and ANG may be involved in the autocrine regulation of the growth and proliferation of pulmonary giant cell carcinoma, TGF alpha and IL-6 may play an important role in the metastasis of the tumor cells.
研究人肺巨细胞癌两种转移变异体PGbE1和PGLH7中某些生长因子的表达及反应差异，以及这些生长因子对肿瘤细胞转移的作用。
采用逆转录-聚合酶链反应（RT-PCR）检测转化生长因子α（TGFα）、转化生长因子β1（TGFβ1）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、碱性成纤维细胞生长因子（bFGF）和血管生成素（ANG）的表达，以及表皮生长因子受体（EGFR）、IL-6受体（IL-6R）和IL-8受体（IL-8R）的表达；采用3H-胸腺嘧啶核苷掺入法测定重组TGFα、TGFβ1和IL-6对两种细胞增殖的影响。
PGbE1细胞中TGFα、EGFR、IL-6和IL-6R的表达水平高于PGLH7细胞。两种细胞中TGFβ1、bFGF、IL-8、IL-8R和ANG的表达无显著差异。重组TGFα和IL-6刺激两种细胞的增殖，而TGFβ1具有双重作用。
TGFα、TGFβ1、bFGF、IL-6、IL-8和ANG可能参与肺巨细胞癌生长和增殖的自分泌调节，TGFα和IL-6可能在肿瘤细胞转移中起重要作用。
