来自颈上神经节的培养大鼠交感神经元中烟碱受体的动力学特性。
Kinetic properties of nicotinic receptors in cultured rat sympathetic neurons from superior cervical ganglia.
作者信息
Zheng J Q, He X P, Yang A Z, Liu C G
机构信息
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 Jul;18(4):330-3.
AIM
To analyze the kinetic properties of the effect of nicotine on nicotinic acetylcholine receptors (nAChR) in the cultured sympathetic neurons from neonatal rat superior cervical ganglia (SCG).
METHODS
The whole-cell recording method of patch-clamp technique was used to record the currents induced by different concentrations of nicotine. The concentration-response of nicotine was fitted with Clark equation.
RESULTS
Hill coefficient (1.097) was determined by fitting the nicotine responses of neuronal nAChR with Clark equation. The theoretical values of nicotine effect, calculated with Clark equation with H = 1, were basically identical with the practically recorded currents.
CONCLUSIONS
Interaction of nicotine and nAChR in rat SCG fits a single binding site model.
目的
分析尼古丁对新生大鼠颈上神经节（SCG）培养的交感神经元中烟碱型乙酰胆碱受体（nAChR）作用的动力学特性。
方法
采用膜片钳技术的全细胞记录方法记录不同浓度尼古丁诱导的电流。尼古丁的浓度-反应关系用克拉克方程拟合。
结果
用克拉克方程拟合神经元nAChR的尼古丁反应，确定希尔系数为1.097。用H = 1的克拉克方程计算的尼古丁效应理论值与实际记录的电流基本相同。
结论
大鼠SCG中尼古丁与nAChR的相互作用符合单结合位点模型。
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