[培养的大鼠颈上神经节神经元的烟碱电流及美加明的使用依赖性阻断]
[Nicotinic currents of cultured rat superior cervical ganglion neurons and use-dependent block by mecamylamine].
作者信息
Liu Q S, He X P, Liu C G
机构信息
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 Nov;16(6):520-3.
AIM
Comparison of action of nicotinic agonists and antagonists on nicotinic acetylcholine receptor (nAChR) in superior cervical ganglion (SCG) neurons.
METHODS
Whole-cell recordings were made from cultured neonatal rat SCG neurons. Cholinergic drugs were applied by local pressure perfusion.
RESULTS
The neurons were activated by nicotinic agonists and peak current were acetylcholine (ACh), 443 +/- 183 pA; nicotine, 1175 +/- 377 pA; dimethylphenylpiperazinium (DMPP), 2946 +/- 358 pA, respectively. The nicotinic responses were blocked by mecamylamine (Mec), hexamethonium and curare, the efficacies were 435 +/- 154 pA, 725 +/- 320 pA, 887 +/- 214 pA, but not by alpha-bungarotoxin. The block by Mec was use-dependent, i.e., it was dependent on repeated presentation of the agonists. The first 6 peak currents were expressed as percentage of the first response as following: 100, 64 +/- 3, 50 +/- 3, 41 +/- 4, 32 +/- 3%.
CONCLUSION
The present data suggest that nAChR of SCG neurons have different pharmacological characteristics from that of muscle or central neurons.
目的
比较烟碱激动剂和拮抗剂对上颈神经节（SCG）神经元烟碱型乙酰胆碱受体（nAChR）的作用。
方法
采用新生大鼠SCG神经元培养物进行全细胞记录。通过局部压力灌注应用胆碱能药物。
结果
神经元被烟碱激动剂激活，峰值电流分别为乙酰胆碱（ACh）443±183 pA、尼古丁1175±377 pA、二甲基苯基哌嗪（DMPP）2946±358 pA。烟碱反应被美加明（Mec）、六甲铵和箭毒阻断，效能分别为435±154 pA、725±320 pA、887±214 pA，但不被α-银环蛇毒素阻断。Mec的阻断具有使用依赖性，即它依赖于激动剂的重复呈现。前6个峰值电流表示为第一个反应的百分比，如下所示：100、64±3、50±3、41±4、32±3%。
结论
目前的数据表明，SCG神经元的nAChR具有与肌肉或中枢神经元不同的药理学特性。
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