环孢素抑制HL-60细胞的钙介导凋亡。
Cyclosporine inhibited calcium-mediated apoptosis of HL-60 cells.
作者信息
Huang Q Q, Fang M, Zhang H Q, Xue S B
机构信息
Department of Biology, Beijing Normal University, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 May;18(3):262-6.
AIM
To study the effects of cyclosporine (Cyc) on apoptosis of HL-60 cells.
METHODS
Apoptotic cells induced by harringtonine (Har), camptothecin (Cam), or calcimycin (Cal), thapsigargin (Tha) were identified with DNA electrophoresis, morphology, and flow cytometry. Relative [Ca2+]i alteration of apoptotic HL-60 cells were determined with flow cytometry.
RESULTS
Cal 1 mg.L-1 or Tha 0.5 mg.L-1 induced apoptosis of HL-60 cells. This effect was inhibited by nontoxic concentration of Cyc 1 mg.L-1. Cyc did not inhibit Har- or Cam-induced apoptosis of HL-60 cells. Both Cal and Tha increased intracellular calcium, whereas Har or Cam did not.
CONCLUSION
Cyc inhibited apoptosis only induced by calcium increasement in HL-60 cells. The mechanism of apoptosis induced by Cal or Tha was different from that by Har or Cam.
目的
研究环孢素（Cyc）对HL-60细胞凋亡的影响。
方法
通过DNA电泳、形态学和流式细胞术鉴定由高三尖杉酯碱（Har）、喜树碱（Cam）、或A23187（Cal）、毒胡萝卜素（Tha）诱导的凋亡细胞。用流式细胞术测定凋亡HL-60细胞的相对细胞内钙离子浓度（[Ca2+]i）变化。
结果
1mg.L-1的Cal或0.5mg.L-1的Tha诱导HL-60细胞凋亡。这种效应被1mg.L-1的无毒浓度Cyc抑制。Cyc不抑制Har或Cam诱导的HL-60细胞凋亡。Cal和Tha均增加细胞内钙，而Har或Cam则不然。
结论
Cyc仅抑制HL-60细胞中由钙增加诱导的凋亡。Cal或Tha诱导凋亡的机制与Har或Cam不同。
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