槲皮素诱导人白血病HL-60细胞凋亡。
Quercetin induced apoptosis in human leukemia HL-60 cells.
作者信息
Xiao D, Zhu S P, Gu Z L
机构信息
Department of Pharmacology, Suzhou Medical College, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 May;18(3):280-3.
AIM
To examine whether quercetin (Que) might induce apoptosis in human leukemia HL-60 cells.
METHODS
DNA fragmentation was visualized by agarose gel electrophoresis. Inhibition of proliferation was measured with a colorimetric MTT-assay. The DNA degradation was determined using flow cytometry, and the microscopic changes were observed by an electron microscope.
RESULTS
Que 15-120 mumol.L-1 elicited typical apoptosis morphological changes including condensed chromatin, nuclear fragmentation, and reduction in volume. DNA fragmentation and DNA degradation in a concentration-dependent manner in HL-60 cells. Que inhibited HL-60 cell proliferation. The values of IC50 and 95% confidence limits were 43 (30-61) mumol.L-1 after 48-h treatment with Que.
CONCLUSION
Que induced apoptosis in HL-60 cells.
目的
研究槲皮素（Que）是否可诱导人白血病HL-60细胞凋亡。
方法
通过琼脂糖凝胶电泳观察DNA片段化。采用比色法MTT检测法测定增殖抑制情况。使用流式细胞术测定DNA降解情况，并用电子显微镜观察微观变化。
结果
15 - 120μmol·L-1的Que引发了典型的凋亡形态学变化，包括染色质浓缩、核碎裂和体积减小。HL-60细胞中DNA片段化和DNA降解呈浓度依赖性。Que抑制HL-60细胞增殖。用Que处理48小时后，IC50值和95%置信限为43（30 - 61）μmol·L-1。
结论
Que可诱导HL-60细胞凋亡。
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