肿瘤坏死因子介导牛脑微血管内皮细胞释放血小板衍生生长因子。

Tumor necrosis factor mediated release of platelet-derived growth factor from bovine cerebral microvascular endothelial cells.

作者信息

Li J, Zhang M, Rui Y C

机构信息

Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai, China.

出版信息

Zhongguo Yao Li Xue Bao. 1997 Mar;18(2):133-6.

PMID:10072964

Abstract

AIM

To study the effect of tumor necrosis factor (TNF) on platelet-derived growth factor (PDGF) release from bovine cerebral microvascular endothelial cells (BCMEC), and the protection of drugs on proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC).

METHODS

BCMEC and BCMSMC were cultured in vitro. Effects of TNF on PDGF release from BCMEC were investigated by crystal violet assay.

RESULTS

TNF did not promote the proliferation of BCMSMC directly. But the postculture medium of BCMEC stimulated by TNF promoted the proliferation of BCMSMC in a concentration (5-20 micrograms.L-1)-dependent manner, and the mitogenic activity was neutralized by antibody to PDGF. The BCMSMC proliferation was increased 34 +/- 4% over control by TNF (20 micrograms.L-1). Imperatorin (Imp), iso-imperatorin (iso-Imp), and 6-(alpha, alpha-phenylacetylpiperazinyl) phenyl-5-methyl-4, 5-dihydro-3 (2H)-pyridazinone (PMDP) 1-100 mumol.L-1 did not affect the PDGF release from BCMEC stimulated by TNF, but inhibited the BCMSMC proliferation promoted by PDGF concentration-dependently.

CONCLUSION

TNF promoted PDGF release from BCMEC. The proliferation of BCMSMC stimulated by TNF must be mediated by growth factor, such as PDGF. Imp, iso-Imp, PMDP inhibited proliferation of BCMSMC stimulated by PDGF.

摘要

目的

研究肿瘤坏死因子（TNF）对牛脑微血管内皮细胞（BCMEC）释放血小板衍生生长因子（PDGF）的影响，以及药物对牛脑微血管平滑肌细胞（BCMSMC）增殖的保护作用。

方法

体外培养BCMEC和BCMSMC。采用结晶紫测定法研究TNF对BCMEC释放PDGF的影响。

结果

TNF不能直接促进BCMSMC的增殖。但经TNF刺激后的BCMEC培养上清液能以浓度（5 - 20微克·升-1）依赖的方式促进BCMSMC的增殖，且促有丝分裂活性可被抗PDGF抗体中和。TNF（20微克·升-1）使BCMSMC的增殖较对照组增加34±4%。欧前胡素（Imp）、异欧前胡素（iso-Imp）和6 -（α，α-苯乙酰哌嗪基）苯基-5 -甲基-4,5 -二氢-3（2H）-哒嗪酮（PMDP）1 - 100微摩尔·升-1不影响TNF刺激的BCMEC释放PDGF，但能浓度依赖性地抑制由PDGF促进的BCMSMC增殖。