Tumor necrosis factor mediated release of platelet-derived growth factor from bovine cerebral microvascular endothelial cells.

AIM

To study the effect of tumor necrosis factor (TNF) on platelet-derived growth factor (PDGF) release from bovine cerebral microvascular endothelial cells (BCMEC), and the protection of drugs on proliferation of bovine cerebral microvascular smooth muscle cells (BCMSMC).

METHODS

BCMEC and BCMSMC were cultured in vitro. Effects of TNF on PDGF release from BCMEC were investigated by crystal violet assay.

RESULTS

TNF did not promote the proliferation of BCMSMC directly. But the postculture medium of BCMEC stimulated by TNF promoted the proliferation of BCMSMC in a concentration (5-20 micrograms.L-1)-dependent manner, and the mitogenic activity was neutralized by antibody to PDGF. The BCMSMC proliferation was increased 34 +/- 4% over control by TNF (20 micrograms.L-1). Imperatorin (Imp), iso-imperatorin (iso-Imp), and 6-(alpha, alpha-phenylacetylpiperazinyl) phenyl-5-methyl-4, 5-dihydro-3 (2H)-pyridazinone (PMDP) 1-100 mumol.L-1 did not affect the PDGF release from BCMEC stimulated by TNF, but inhibited the BCMSMC proliferation promoted by PDGF concentration-dependently.

CONCLUSION

TNF promoted PDGF release from BCMEC. The proliferation of BCMSMC stimulated by TNF must be mediated by growth factor, such as PDGF. Imp, iso-Imp, PMDP inhibited proliferation of BCMSMC stimulated by PDGF.