4-氧代和4-羟基氮氧化物在大鼠组织和红细胞中的抗氧化活性。

Antioxidative activity of 4-oxy- and 4-hydroxy-nitroxides in tissues and erythrocytes from rats.

作者信息

Wu Y J, Li W G, Zhang Z M, Tian X

机构信息

Department of Pharmacology, Lanzhou Medical College, China.

出版信息

Zhongguo Yao Li Xue Bao. 1997 Mar;18(2):150-4.

PMID:10072969

Abstract

AIM

To compare the activities of antioxidation of 4-oxy- and 4-hydroxy-nitroxides in tissues and RBC from rats.

METHODS

The homogenates of liver, heart, and kidneys of rats were used to determine malondialdehyde (MDA) formation using TBA colorimetric method. H2O2-caused hemolysis was measured spectrometrically. Superoxide anion from zymosan-stimulated neutrophils of rats was assayed by NBT reduction method.

RESULTS

Nitroxide free radicals OTMPO and HTMPO inhibited MDA generation caused by .OH generation system (MIC 10.5 and 21 mumol.L-1, respectively), antagonized hemolysis induced by H2O2 (MIC: 338 and 168 mumol.L-1, respectively), but did not affect O2- formation from activated neutrophils. 1-Hydroxyl compounds OTMPOH and HTMPOH possessed similarly potent antilipoperoxidative activities. But nonfree radical OTMP and HTMP had no effect on peroxidation of tissues.

CONCLUSION

Nitroxides exert their antilipoperoxidative effect by specifically scavenging .OH free radicals in biological system. Trapping of .OH free radicals by nitroxides is not by reduction of NO. group in nitroxides. Both NO. group and NOH group are essential active groups.

摘要

目的

比较4-氧代和4-羟基氮氧化物在大鼠组织和红细胞中的抗氧化活性。

方法

用大鼠肝脏、心脏和肾脏的匀浆，采用硫代巴比妥酸比色法测定丙二醛（MDA）的生成。用分光光度法测定过氧化氢诱导的溶血。用NBT还原法测定大鼠酵母聚糖刺激的中性粒细胞产生的超氧阴离子。

结果

氮氧自由基OTMPO和HTMPO抑制由·OH生成系统引起的MDA生成（MIC分别为10.5和21μmol·L-1），拮抗过氧化氢诱导的溶血（MIC分别为338和168μmol·L-1），但不影响活化中性粒细胞产生O2-。1-羟基化合物OTMPOH和HTMPOH具有同样强的抗脂质过氧化活性。但非自由基OTMP和HTMP对组织过氧化无影响。