环己酰亚胺不能阻止α-双炔失碳酯诱导的白血病K562细胞凋亡。
alpha-Anordrin-induced apoptosis of leukemia K562 cells is not prevented by cicloheximide.
作者信息
Lou L G, Xu B
机构信息
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 Mar;18(2):169-72.
AIM
To study effect of protein synthesis inhibitor cicloheximide (Cic) on the apoptosis induced by alpha-anordrin (Ano) in leukemia K562 cells.
METHODS
Morphological changes were observed by fluorescent microscopy. DNA content was measured by flow cytometry. DNA fragmentation was analyzed by agarose gel electrophoresis.
RESULTS
Exposure of K562 cells to Ano 50 mumol.L-1 for 24 h induced apoptotic cell death. Cic 1 mumol.L-1 did not abrogate or delay this effect. Indeed, Ano-induced apoptosis was augmented by Cic. Cic 100 mumol.L-1 itself stimulated 25% K562 cell apoptosis after 24-h culture.
CONCLUSION
Ano-induced apoptosis was independent of de novo protein synthesis.
目的
研究蛋白质合成抑制剂环己酰亚胺（Cic）对α-双炔失碳酯（Ano）诱导白血病K562细胞凋亡的影响。
方法
通过荧光显微镜观察形态学变化。采用流式细胞术检测DNA含量。用琼脂糖凝胶电泳分析DNA片段化。
结果
K562细胞暴露于50μmol.L-1的Ano中24小时可诱导凋亡性细胞死亡。1μmol.L-1的Cic不会消除或延迟这种效应。实际上，Cic增强了Ano诱导的凋亡。100μmol.L-1的Cic在培养24小时后自身可刺激25%的K562细胞凋亡。
结论
Ano诱导的凋亡与从头合成蛋白质无关。
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