Masson P
Centre de Recherches du Service de Santé des Armées Emile Pardé, Département de Toxicologie, La Tronche.
Ann Pharm Fr. 1999 Jan;57(1):49-55.
High pressure engineering of proteins is an emerging biotechnology. The role of pressure in the control of protein stability and reactivity is reviewed. It is shown that a better understanding of the behavior of proteins under extreme pressure and temperature conditions may help to improve biochemical processes under non conventional medial/conditions for enzyme-catalyzed production of fine chemicals and the use of modified proteins of medical or pharmaceutical interest. Moreover, high pressure can be used instead of heart or chemicals for sterilization.
蛋白质的高压工程是一种新兴的生物技术。本文综述了压力在控制蛋白质稳定性和反应活性方面的作用。结果表明，更好地理解蛋白质在极端压力和温度条件下的行为，可能有助于改善在非传统介质/条件下进行酶催化精细化学品生产以及利用具有医学或药学意义的修饰蛋白质的生化过程。此外，高压可替代热或化学物质用于灭菌。
