Nijhar S K, Mackay D K, Brocchi E, Ferris N P, Kitching R P, Knowles N J
J Gen Virol. 1999 Feb;80 ( Pt 2):277-282. doi: 10.1099/0022-1317-80-2-277.
Six neutralizing monoclonal antibodies (MAbs) were used to isolate MAb neutralization-resistant (MAR) mutants from a recent European strain of swine vesicular disease virus (SVDV), ITL/9/93. Sequencing of MAR mutants identified two epitopes located at positions analogous to sites 2A (VP2) and 3B (VP3) on poliovirus (PV) which have been previously identified on a Japanese strain of SVDV. A third epitope near to the C terminus of VP1, not previously recognized on SVDV, was tentatively identified in a region analogous to site 1 of PV. A fourth epitope, located in the C-terminal region of VP3, has never before been recognized as a site of neutralization on picornaviruses. All four epitopes were predicted to be surface-exposed.
使用六种中和单克隆抗体（MAb）从猪水疱病病毒（SVDV）的一株近期欧洲毒株ITL/9/93中分离出单克隆抗体中和抗性（MAR）突变体。对MAR突变体进行测序，确定了两个表位，其位置类似于脊髓灰质炎病毒（PV）上的2A（VP2）和3B（VP3）位点，此前在一株日本SVDV毒株上已鉴定出这两个位点。在类似于PV位点1的区域初步鉴定出第三个表位，该表位靠近VP1的C末端，此前在SVDV上未被识别。第四个表位位于VP3的C末端区域，以前从未被认为是小RNA病毒的中和位点。预计所有四个表位都暴露于表面。
