Xu Wanhong, Goolia Melissa, Salo Tim, Zhang Zhidong, Yang Ming
National Centre for Foreign Animal Disease, Winnipeg, MB R3E 3M4, Canada.
J Vet Sci. 2017 Aug 31;18(S1):361-370. doi: 10.4142/jvs.2017.18.S1.361.
Swine vesicular disease (SVD) is a highly contagious viral disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from those of foot-and-mouth disease, which prevents international trade of swine and related products. SVD-specific antibody detection via an enzyme-linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. Inactivated SVD virus is the commonly used antigen in SVD-related ELISA. A recombinant SVD virus-like particle (VLP) was generated by using a Bac-to-Bac baculovirus expression system. Results of SVD-VLP analyses from electron microscopy, western blotting, immunofluorescent assay, and mass spectrometry showed that the recombinant SVD-VLP morphologically resemble authentic SVD viruses. The SVD-VLP was evaluated as a replacement for inactivated whole SVD virus in competitive and isotype-specific ELISAs for the detection of antibodies against SVD virus. The recombinant SVD-VLP assay produced results similar to those from inactivated whole virus antigen ELISA. The VLP-based ELISA results were comparable to those from the virus neutralization test for antibody detection in pigs experimentally inoculated with SVD virus. Use of the recombinant SVD-VLP is a safe and valuable alternative to using SVD virus antigen in diagnostic assays.
猪水泡病（SVD）是一种高度传染性的病毒性疾病，可导致猪的水泡病。该疾病的重要性在于其临床症状与口蹄疫难以区分，这阻碍了猪及相关产品的国际贸易。通过酶联免疫吸附测定（ELISA）检测SVD特异性抗体是SVD监测和出口认证中最通用且常用的方法。灭活的SVD病毒是SVD相关ELISA中常用的抗原。利用杆状病毒表达系统Bac-to-Bac产生了重组SVD病毒样颗粒（VLP）。电子显微镜、蛋白质印迹、免疫荧光测定和质谱分析SVD-VLP的结果表明，重组SVD-VLP在形态上类似于真实的SVD病毒。在用于检测抗SVD病毒抗体的竞争型和同型特异性ELISA中，评估了SVD-VLP作为灭活的完整SVD病毒的替代品。重组SVD-VLP检测产生的结果与灭活全病毒抗原ELISA的结果相似。基于VLP的ELISA结果与在实验性接种SVD病毒的猪中进行抗体检测的病毒中和试验结果相当。在诊断检测中，使用重组SVD-VLP是使用SVD病毒抗原的一种安全且有价值的替代方法。
