Buck M, Cross S, Krauer K, Kienzle N, Sculley T B
J Gen Virol. 1999 Feb;80 ( Pt 2):441-445. doi: 10.1099/0022-1317-80-2-441.
In this study replication of A-type and B-type Epstein-Barr virus (EBV) strains has been assessed. A-type and B-type type lymphoblastoid cell lines (LCLs) were established by infecting B lymphocytes, isolated from five EBV-seropositive donors, with different A-type and B-type virus isolates. The presence of viral capsid antigens (VCA) in these LCLs was determined by immunofluoresence assay and by immunoblotting. All of the B-type EBV strains were capable of spontaneously generating virus regardless of the origin of the donor cells. In contrast the A-type strains, other than strain IARC-BL36, did not readily produce VCA in any of the different donor lymphocytes used. This study demonstrates another biological difference between the two virus types: their ability to spontaneously enter the lytic cycle.
在本研究中，对A 型和B 型爱泼斯坦-巴尔病毒（EBV）毒株的复制情况进行了评估。通过用不同的A 型和B 型病毒分离株感染从五名EBV 血清阳性供体中分离出的B 淋巴细胞，建立了A 型和B 型淋巴母细胞样细胞系（LCL）。通过免疫荧光测定法和免疫印迹法确定这些LCL 中病毒衣壳抗原（VCA）的存在。所有B 型EBV 毒株都能够自发产生病毒，而不论供体细胞的来源如何。相比之下，除IARC-BL36 毒株外，A 型毒株在所用的任何不同供体淋巴细胞中都不容易产生VCA。这项研究证明了这两种病毒类型之间的另一个生物学差异：它们自发进入裂解周期的能力。
