探讨智利南部胃癌患者中 EB 病毒的遗传多样性。
Exploring the Genetic Diversity of Epstein-Barr Virus among Patients with Gastric Cancer in Southern Chile.
机构信息
Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Temuco 4810101, Chile.
Doctorado en Ciencias Médicas, Universidad de La Frontera, Temuco 4811230, Chile.
出版信息
Int J Mol Sci. 2023 Jul 10;24(14):11276. doi: 10.3390/ijms241411276.
The Epstein-Barr virus (EBV) has been associated with gastric cancer (GC), one of the deadliest malignancies in Chile and the world. Little is known about Chilean EBV strains. This study aims to investigate the frequency and genetic diversity of EBV in GC in patients in southern Chile. To evaluate the prevalence of EBV in GC patients from the Chilean population, we studied 54 GC samples using the gold standard detection method of EBV-encoded small RNA (EBER). The EBV-positive samples were subjected to amplification and sequencing of the Epstein-Barr virus nuclear protein 3A () gene to evaluate the genetic diversity of EBV strains circulating in southern Chile. In total, 22.2% of the GC samples were EBV-positive and significantly associated with diffuse-type histology ( = 0.003). Phylogenetic analyses identified EBV-1 and EBV-2 in the GC samples, showing genetic diversity among Chilean isolates. This work provides important information for an epidemiological follow-up of the different EBV subtypes that may cause GC in southern Chile.
爱泼斯坦-巴尔病毒（EBV）与胃癌（GC）有关，胃癌是智利和全球最致命的恶性肿瘤之一。关于智利 EBV 株知之甚少。本研究旨在调查智利南部地区胃癌患者中 EBV 的频率和遗传多样性。为了评估 EBV 在智利人群中 GC 患者中的流行情况，我们使用 EBV 编码的小 RNA（EBER）的金标准检测方法研究了 54 个 GC 样本。对 EBV 阳性样本进行 Epstein-Barr 病毒核蛋白 3A()基因的扩增和测序，以评估在智利南部流行的 EBV 株的遗传多样性。总共，22.2%的 GC 样本 EBV 阳性，与弥漫型组织学显著相关（=0.003）。系统进化分析在 GC 样本中鉴定出 EBV-1 和 EBV-2，显示出智利分离株之间的遗传多样性。这项工作为不同 EBV 亚型在智利南部引起 GC 的流行病学随访提供了重要信息。
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