双生病毒菜豆黄矮病毒适应双子叶植物宿主涉及病毒链和互补链基因。

Adaptation of the geminivirus bean yellow dwarf virus to dicotyledonous hosts involves both virion-sense and complementary-sense genes.

作者信息

Liu L, Pinner M S, Davies J W, Stanley J

出版信息

J Gen Virol. 1999 Feb;80 ( Pt 2):501-506. doi: 10.1099/0022-1317-80-2-501.

Abstract

Bean yellow dwarf virus (BeYDV) and maize streak virus (MSV) belong to the geminivirus genus Mastrevirus and have host ranges confined to dicotyledonous and monocotyledonous species, respectively. To investigate viral determinants of host range specificity, chimeras were constructed by exchanging their coding and non-coding regions. BeYDV chimeras containing MSV ORF V1, ORF V2 or small intergenic region sequences, either individually or in various sequential combinations, replicated and produced virus particles in Nicotiana tabacum protoplasts. BeYDV chimeras containing MSV ORFs C1 and C2 and/or the large intergenic region were unable to replicate. None of the chimeras was able to systemically infect either N. benthamiana or maize. Complementation experiments using BeYDV chimeras containing MSV ORF V1 and/or ORF V2 suggest that expression of MSV movement protein and/or coat protein prevents BeYDV movement. The results demonstrate that factors involved in both viral DNA replication and virus movement are exclusively adapted to either monocotyledonous or dicotyledonous host backgrounds.

摘要

菜豆黄矮病毒（BeYDV）和玉米条纹病毒（MSV）属于双生病毒属的玉米线条病毒属，其宿主范围分别局限于双子叶植物和单子叶植物。为了研究宿主范围特异性的病毒决定因素，通过交换它们的编码区和非编码区构建了嵌合体。含有MSV ORF V1、ORF V2或小基因间隔区序列的BeYDV嵌合体，无论是单独还是以各种连续组合形式，都能在烟草原生质体中复制并产生病毒粒子。含有MSV ORFs C1和C2和/或大基因间隔区的BeYDV嵌合体无法复制。这些嵌合体均不能系统感染本氏烟草或玉米。使用含有MSV ORF V1和/或ORF V2的BeYDV嵌合体进行的互补实验表明，MSV运动蛋白和/或外壳蛋白的表达会阻止BeYDV移动。结果表明，参与病毒DNA复制和病毒移动的因素仅适应单子叶或双子叶宿主背景。