毒蝇伞苯丙氨酸解氨酶基因的糖和氮依赖性调控
Sugar- and nitrogen-dependent regulation of an Amanita muscaria phenylalanine ammonium lyase gene.
作者信息
Nehls U, Ecke M, Hampp R
机构信息
Universität Tübingen, Botanisches Institut, Physiologische Okologie der Pflanzen, 72076 Tübingen, Germany.
出版信息
J Bacteriol. 1999 Mar;181(6):1931-3. doi: 10.1128/JB.181.6.1931-1933.1999.
Abstract
The cDNA of a key enzyme of secondary metabolism, phenylalanine ammonium lyase, was identified for an ectomycorrhizal fungus by differential screening of a mycorrhizal library. The gene was highly expressed in hyphae grown at low external monosaccharide concentrations, but its expression was 30-fold reduced at elevated concentrations. Gene repression was regulated by hexokinase.
摘要
通过对菌根文库的差异筛选，鉴定出一种外生菌根真菌次生代谢关键酶苯丙氨酸解氨酶的cDNA。该基因在低外源单糖浓度下生长的菌丝中高度表达，但在浓度升高时其表达降低了30倍。基因抑制受己糖激酶调控。
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