Zhang L, Li X, Shong X, Tian N
Lab of Microcirculation, Chinese PLA General Hospital, Beijing.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 1997 Feb;13(1):1-4.
Intercellular adhesion molecule-1 (ICAM-1) expression was studied with rat transient cerebral ischemic model. The results showed that the expression of ICAM-1 markedly increased after 1 h ischemia following 6 h reperfusion. Laser confocal microscope demonstrated that the FITC quantum on blood vessels after reperfusion was more than 47% compared to that of pure ischemia. MPO activity and light microscope observation showed that leukocytes accumulated in injured tissue 9 h after reperfusion. The local IL-1 content in brain tissue changed with different period of reperfusion time. Our data indicated that after brain ischemia-reperfusion injury ICAM-1 expression was in time-dependent increase, the local IL-1 secretion might up regulate the ICAM-1 expression, there were large amount of leukocytes accumulated in surrounding tissue, the increase of ICAM-1 expression was the prerequisite for leukocyte adhesion and migration.
采用大鼠短暂性脑缺血模型研究细胞间黏附分子-1（ICAM-1）的表达。结果显示，缺血6小时再灌注1小时后，ICAM-1的表达显著增加。激光共聚焦显微镜显示，再灌注后血管上的异硫氰酸荧光素（FITC）量比单纯缺血时多47%以上。髓过氧化物酶（MPO）活性及光学显微镜观察显示，再灌注9小时后白细胞在损伤组织中积聚。脑组织局部白细胞介素-1（IL-1）含量随再灌注时间的不同时期而变化。我们的数据表明，脑缺血再灌注损伤后ICAM-1表达呈时间依赖性增加，局部IL-1分泌可能上调ICAM-1表达，周围组织中有大量白细胞积聚，ICAM-1表达增加是白细胞黏附和迁移的前提条件。
