改良BACTEC 12B放射测量培养基和固体培养基用于绵羊副结核分枝杆菌培养的评估。
Evaluation of modified BACTEC 12B radiometric medium and solid media for culture of Mycobacterium avium subsp. paratuberculosis from sheep.
作者信息
Whittington R J, Marsh I, McAllister S, Turner M J, Marshall D J, Fraser C A
机构信息
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia.
出版信息
J Clin Microbiol. 1999 Apr;37(4):1077-83. doi: 10.1128/JCM.37.4.1077-1083.1999.
Definitive diagnosis of Johne's disease in ruminants depends on confirming the presence of the causative bacterium, Mycobacterium avium subsp. paratuberculosis, in tissues of the host. This is readily achieved in most ruminant species by culture. However, culture of clinical specimens from sheep in many countries has been unrewarding. Such a culture from sheep was achieved recently in Australia by using a radiometric culture medium. The aims of the present study were to evaluate the culture of M. avium subsp. paratuberculosis from sheep by using modified BACTEC 12B radiometric medium, to determine the sensitivity of culture in relation to histopathology, and to evaluate a range of solid media. Culture of M. avium subsp. paratuberculosis from sheep with Johne's disease is a sensitive method of diagnosis: intestinal tissues from all 43 animals with multibacillary disease and all 22 animals with paucibacillary disease were culture positive, while 98% of feces from 53 animals with multibacillary disease and 48% of feces from 31 animals with paucibacillary disease were culture positive. Of sheep without histological evidence of Johne's disease from infected flocks, intestinal tissue from 32% of 41 were culture positive, while feces from 17% of 41 were culture positive. Consequently, culture is recommended as the "gold standard" test for detection of ovine Johne's disease. Of the wide range of solid media that were evaluated, only modified Middlebrook 7H10 and 7H11 agars, which were very similar in composition to modified BACTEC 12B medium, yielded growth of ovine strains of M. avium subsp. paratuberculosis. The sensitivity of detection of M. avium subsp. paratuberculosis on solid media was slightly lower than that in modified BACTEC 12B radiometric medium. Both egg yolk and mycobactin J were essential additives for growth of ovine strains of M. avium subsp. paratuberculosis in both liquid and solid media.
反刍动物若尼氏病的确切诊断取决于在宿主体组织中确认致病细菌副结核分枝杆菌的存在。在大多数反刍动物物种中，通过培养很容易做到这一点。然而，在许多国家，从绵羊的临床标本中进行培养一直没有收获。最近在澳大利亚，通过使用放射性培养基从绵羊身上实现了这样的培养。本研究的目的是评估使用改良的BACTEC 12B放射性培养基对绵羊副结核分枝杆菌的培养，确定培养相对于组织病理学的敏感性，并评估一系列固体培养基。从患有若尼氏病的绵羊中培养副结核分枝杆菌是一种敏感的诊断方法：所有43只患有多菌型疾病的动物和所有22只患有少菌型疾病的动物的肠道组织培养均呈阳性，而53只患有多菌型疾病的动物中98%的粪便和31只患有少菌型疾病的动物中48%的粪便培养呈阳性。在来自受感染羊群但无若尼氏病组织学证据的绵羊中，41只中有32%的肠道组织培养呈阳性，而41只中有17%的粪便培养呈阳性。因此，建议将培养作为检测绵羊若尼氏病的“金标准”试验。在所评估的广泛的固体培养基中，只有成分与改良的BACTEC 12B培养基非常相似的改良Middlebrook 7H10和7H11琼脂培养出了绵羊副结核分枝杆菌菌株。在固体培养基上检测副结核分枝杆菌的敏感性略低于在改良的BACTEC 12B放射性培养基中的敏感性。蛋黄和分枝杆菌素J都是绵羊副结核分枝杆菌菌株在液体和固体培养基中生长所必需的添加剂。
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