Diserens H, Henzelin M
NESTEC Ltd., Nestlé Research Centre, Lausanne, Switzerland.
J Chromatogr A. 1999 Feb 12;833(1):13-8. doi: 10.1016/s0021-9673(98)00863-2.
A rapid and sensitive HPLC method has been developed and validated for the determination of abamectin residues (avermectin B1a and B1b, as well as the metabolite 8,9-Z-avermectin B1) in apples, pears and tomatoes. Residues are extracted with acetonitrile. The diluted extract is cleaned up on a C18 solid-phase extraction cartridge. Abamectin residues are derivatised with trifluoroacetic acid and 1-methylimidazole and determined by reversed-phase liquid chromatography with fluorescence detection (excitation: 365 nm and emission: 470 nm). High and consistent recoveries, ranging from 88 to 106%, were obtained, at spiking levels of 10, 20 and 50 micrograms/kg, when analysing apples, pears and tomatoes.
已开发并验证了一种快速灵敏的高效液相色谱法，用于测定苹果、梨和番茄中的阿维菌素残留量（阿维菌素B1a和B1b以及代谢物8,9-Z-阿维菌素B1）。残留量用乙腈提取。稀释后的提取物在C18固相萃取柱上进行净化。阿维菌素残留量用三氟乙酸和1-甲基咪唑衍生化，并用荧光检测（激发波长：365nm，发射波长：470nm）的反相液相色谱法测定。在对苹果、梨和番茄进行分析时，当添加水平为10、20和50微克/千克时，获得了88%至106%的高且一致的回收率。
