稻米中阿维菌素、甲氨基阿维菌素苯甲酸盐和伊维菌素多残留的简化反相高效液相色谱分析方法。

Simplified RP-HPLC method for multi-residue analysis of abamectin, emamectin benzoate and ivermectin in rice.

机构信息

State Key Laboratory of Pollution Control and Resource Reuse, Center for Hydrosciences Research, Nanjing University, School of the Environment, Nanjing 210093, China.

出版信息

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Jan;28(1):19-25. doi: 10.1080/19440049.2010.527377.

DOI:10.1080/19440049.2010.527377

PMID:21132591

Abstract

A rapid, reliable and sensitive reverse-phase high-performance liquid chromatography method with fluorescence detection (RP-FLD-HPLC) was developed and validated for simultaneous analysis of the abamectin (ABA), emamectin (EMA) benzoate and ivermectin (IVM) residues in rice. After extraction with acetonitrile/water (2 : 1) with sonication, the avermectin (AVMs) residues were directly derivatised by N-methylimidazole (N-NMIM) and trifluoroacetic anhydride (TFAA) and then analysed on RP-FLD-HPLC. A good linear relationship (r(2 )> 0.99) was obtained for three AVMs ranging from 0.01 to 5 microg ml(-1), i.e. 0.01-5.0 microg g(-1) in rice matrix. The limit of detection (LOD) and the limit of quantification (LOQ) were between 0.001 and 0.002 microg g(-1) and between 0.004 and 0.006 microg g(-1), respectively. Recoveries were from 81.9% to 105.4% and precision less than 12.4%. The proposed method was successfully applied to routine analysis of the AVMs residues in rice.

摘要

建立并验证了一种用于稻米中阿维菌素（ABA）、甲氨基阿维菌素苯甲酸盐（EMA）和伊维菌素（IVM）残留量同时分析的快速、可靠和灵敏的反相高效液相色谱法（RP-FLD-HPLC）。经乙腈/水（2:1）超声提取后，阿维菌素（AVMs）残留直接用 N-甲基咪唑（N-NMIM）和三氟乙酸酐（TFAA）衍生，然后在 RP-FLD-HPLC 上进行分析。在稻米基质中，三种 AVMs 的浓度范围为 0.01-5μgml（-1）（0.01-5.0μg g（-1））时，均呈现良好的线性关系（r（2）>0.99）。检测限（LOD）和定量限（LOQ）分别在 0.001-0.002μg g（-1）和 0.004-0.006μg g（-1）之间。回收率为 81.9%-105.4%，精密度小于 12.4%。该方法成功应用于稻米中 AVMs 残留的常规分析。