State Key Laboratory of Pollution Control and Resource Reuse, Center for Hydrosciences Research, Nanjing University, School of the Environment, Nanjing 210093, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Jan;28(1):19-25. doi: 10.1080/19440049.2010.527377.
A rapid, reliable and sensitive reverse-phase high-performance liquid chromatography method with fluorescence detection (RP-FLD-HPLC) was developed and validated for simultaneous analysis of the abamectin (ABA), emamectin (EMA) benzoate and ivermectin (IVM) residues in rice. After extraction with acetonitrile/water (2 : 1) with sonication, the avermectin (AVMs) residues were directly derivatised by N-methylimidazole (N-NMIM) and trifluoroacetic anhydride (TFAA) and then analysed on RP-FLD-HPLC. A good linear relationship (r(2 )> 0.99) was obtained for three AVMs ranging from 0.01 to 5 microg ml(-1), i.e. 0.01-5.0 microg g(-1) in rice matrix. The limit of detection (LOD) and the limit of quantification (LOQ) were between 0.001 and 0.002 microg g(-1) and between 0.004 and 0.006 microg g(-1), respectively. Recoveries were from 81.9% to 105.4% and precision less than 12.4%. The proposed method was successfully applied to routine analysis of the AVMs residues in rice.
建立并验证了一种用于稻米中阿维菌素（ABA）、甲氨基阿维菌素苯甲酸盐（EMA）和伊维菌素（IVM）残留量同时分析的快速、可靠和灵敏的反相高效液相色谱法（RP-FLD-HPLC）。经乙腈/水（2:1）超声提取后，阿维菌素（AVMs）残留直接用 N-甲基咪唑（N-NMIM）和三氟乙酸酐（TFAA）衍生，然后在 RP-FLD-HPLC 上进行分析。在稻米基质中，三种 AVMs 的浓度范围为 0.01-5μgml（-1）（0.01-5.0μg g（-1））时，均呈现良好的线性关系（r（2）>0.99）。检测限（LOD）和定量限（LOQ）分别在 0.001-0.002μg g（-1）和 0.004-0.006μg g（-1）之间。回收率为 81.9%-105.4%，精密度小于 12.4%。该方法成功应用于稻米中 AVMs 残留的常规分析。
