液相色谱-质谱法测定苹果和苹果叶中的比久
Determination of daminozide in apples and apple leaves by liquid chromatography--mass spectrometry.
作者信息
Mol H G, van Dam R C, Vreeken R J, Steijger O M
机构信息
TNO Nutrition and Food Research Institute, Department of Pesticides, Zeist, The Netherlands.
出版信息
J Chromatogr A. 1999 Feb 12;833(1):53-60. doi: 10.1016/s0021-9673(98)00915-7.
A straightforward and efficient method was developed for the determination of intact daminozide in apples and apple leaves. After extraction with methanol and a clean-up step using a graphitized carbon cartridge, the extract was analysed by ion-trap liquid chromatography-tandem mass spectrometry (LC-MS-MS) using atmospheric pressure chemical ionisation in the positive ion mode. Recoveries for apple were 98-102% with a R.S.D. < or = 11% (n = 6) and for leaves were 112-116% with a R.S.D. < or = 18% (n = 6). The limits of detection were 0.008 and 0.02 mg/kg for apples and leaves, respectively.
开发了一种简单高效的方法用于测定苹果和苹果叶中的完整丁酰肼。用甲醇提取并通过石墨化碳柱进行净化步骤后，提取物采用大气压化学电离正离子模式，通过离子阱液相色谱 - 串联质谱法（LC-MS-MS）进行分析。苹果的回收率为98 - 102%，相对标准偏差≤11%（n = 6），叶子的回收率为112 - 116%，相对标准偏差≤18%（n = 6）。苹果和叶子的检测限分别为0.008和0.02 mg/kg。
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