Electrophysiological effects of oxytocin within the bed nuclei of the stria terminalis: influence of reproductive stage and ovarian steroids.

The bed nuclei of the stria terminalis (BNST) is a target site for the central actions of oxytocin (OT) in promoting behavioural and neuroendocrine responses involved in female reproduction, and binding studies suggest that OT sensitivity may be modulated over the peripartum period. Electrophysiological recordings from brain slices in vitro showed that OT sensitivity of BNST neurones is relatively low in late pregnancy, but is high during lactation. In vivo studies over the immediate peri-partum period revealed that although BNST neurones can be excited by i.c.v. OT at day 22 of pregnancy, there is a 5-10 min delay in their response which is not present in lactation. This delay can be reversed by naltrexone, or lesioning the stria terminalis, and may involve an inhibitory opioid input to the BNST from the amygdala. Examination of the role of steroids in regulating OT responses of BNST neurones showed that oestradiol pre-treatment in late pregnant ovariectomized rats increased OT excitation of BNST neurones in vitro, and a similar result was observed with in vivo recordings. Progesterone also augmented OT excitation of BNST neurones in vitro, but no such effect was observed in vivo. This difference could indicate that an additional effect of progesterone is to potentiate extraneous inhibitory inputs to the BNST, or may reflect the ability of this steroid to suppress OT sensitivity by a direct membrane action. Changes in the response of BNST neurones to OT may have functional implications for the action of central OT in facilitating the neuroendocrine milk-ejection reflex (i.e. increasing milk-ejection frequency), an effect which first appears at around day 3 of lactation. Studies involving steroid treatment of late pregnant ovariectomized rats showed that this facilitatory mechanism can be induced to appear early (i.e. on day 22 of pregnancy) by oestradiol, but not progesterone treatment. Collectively, these results support this view, that the action of OT in the BNST is regulated by the changing levels of steroids towards the end of pregnancy, thereby ensuring appropriate neuroendocrine responses necessary for motherhood.