通过毛细管电泳分析生物体液中的亚硝酸盐和硝酸盐。

Analysis of nitrite and nitrate in biological fluids by capillary electrophoresis.

作者信息

Bories P N, Scherman E, Dziedzic L

机构信息

Laboratoire de Biochimie, Hôpital Albert Chenevier, Assistance Publique-Hôpitaux de Paris, Créteil, France.

出版信息

Clin Biochem. 1999 Feb;32(1):9-14. doi: 10.1016/s0009-9120(98)00090-3.

DOI:10.1016/s0009-9120(98)00090-3

PMID:10074886

Abstract

OBJECTIVES

We report a method for determining nitrite and nitrate in biological fluids by capillary electrophoresis.

METHODS

A Waters capillary electrophoresis system was used with a filter for detection at 214 nm. After dilution with distilled water, the sample was loaded hydrostatically onto a 60 cm x 100 microm capillary and electrophoresed at 15 kV in 15 mmol/L sulfate buffer, pH 8.0, containing 2.5% electroosmotic flow modifier.

RESULTS

The retention times for nitrite and nitrate were 3.9 +/- 0.8 and 4.0 +/- 0.8 min, respectively. The detection limit was 10 micromol/L for serum nitrate. The recovery was 93-115% for nitrite and 92-106% for nitrate. The within-day and between-day coefficients of variation were lower than 3.3% and 5.0%, respectively, for two pools with normal (28 micromol/L) and high (87 micromol/L) nitrate concentration. A comparison with the nitrate reductase method gave a correlation coefficient of 0.982.

CONCLUSION

Capillary electrophoresis provides many advantages, namely low cost, small sample and buffer requirements, rapidity, which makes its use particularly suitable for clinical laboratories.

摘要

目的

我们报告一种通过毛细管电泳测定生物体液中亚硝酸盐和硝酸盐的方法。

方法

使用沃特世毛细管电泳系统，配备在214nm处进行检测的检测器。用蒸馏水稀释后，将样品通过静水压加载到一根60cm×100μm的毛细管中，并在含2.5%电渗流改性剂的15mmol/L硫酸盐缓冲液（pH 8.0）中于15kV电压下进行电泳。

结果

亚硝酸盐和硝酸盐的保留时间分别为3.9±0.8分钟和4.0±0.8分钟。血清硝酸盐的检测限为10μmol/L。亚硝酸盐的回收率为93% - 115%，硝酸盐的回收率为92% - 106%。对于硝酸盐浓度正常（28μmol/L）和高（87μmol/L）的两个样本池，日内和日间变异系数分别低于3.3%和5.0%。与硝酸盐还原酶法比较，相关系数为0.982。