Obermair A, Tempfer C, Wasicky R, Kaider A, Hefler L, Kainz C
Department of Gynecology and Obstetrics, University Hospital of Vienna, Austria.
Obstet Gynecol. 1999 Mar;93(3):367-71. doi: 10.1016/s0029-7844(98)00417-7.
To determine the prognostic effect of intratumor microvessel density in a series of unselected patients with endometrial carcinoma.
We reviewed 93 consecutive patients treated surgically for endometrial cancer at the University Hospital of Vienna between 1983 and 1989. Histologic sections were obtained from original paraffin-embedded blocks and stained immunohistochemically for CD34 antigen. Microvessel density was determined by enumeration of intratumor CD34-positive cells under a light microscope at 200 x magnification using an examination area of 0.74 mm2. Log-rank test and Cox proportional-hazards models (univariate and multivariate) were applied for overall survival analysis.
Overall, the 25% quantile of survival was reached at 37.9 months. The 5-year survival rate was 82.2% in 69 patients whose tumors had microvessel counts no more than 100/0.74 mm2 field, and 52.0% in 24 patients whose tumors had microvessel counts of more than 100/0.74 mm2 field (log-rank P = .004). In the multiple Cox model, high microvessel counts (relative risk [RR] 1.2; 95% confidence interval [CI] 1.1, 1.4) as well as undifferentiated tumors (RR 6.1; CI 2.2, 16.8), and advanced stage of disease (RR 2.6; CI 1.3, 5.1) independently exerted an adverse influence on the survival of patients with endometrial cancer.
High intratumor microvessel count is associated with poor survival of patients with endometrial cancer.
确定肿瘤内微血管密度对一系列未经选择的子宫内膜癌患者的预后影响。
我们回顾了1983年至1989年在维也纳大学医院接受手术治疗的93例连续子宫内膜癌患者。从原始石蜡包埋块中获取组织学切片，并进行免疫组织化学染色以检测CD34抗原。在光学显微镜下以200倍放大倍数，使用0.74mm²的检查区域，通过计数肿瘤内CD34阳性细胞来确定微血管密度。应用对数秩检验和Cox比例风险模型（单变量和多变量）进行总生存分析。
总体而言，生存时间的25%分位数为37.9个月。69例肿瘤微血管计数不超过100/0.74mm²视野的患者5年生存率为82.2%，24例肿瘤微血管计数超过100/0.74mm²视野的患者5年生存率为52.0%（对数秩检验P = 0.004）。在多变量Cox模型中，高微血管计数（相对风险[RR] 1.2；95%置信区间[CI] 1.1, 1.4）以及未分化肿瘤（RR 6.1；CI 2.2, 16.8）和疾病晚期（RR 2.6；CI 1.3, 5.1）独立地对子宫内膜癌患者的生存产生不利影响。
肿瘤内微血管计数高与子宫内膜癌患者的不良生存相关。
