酶催化反应在有机介质中的多重强化性能。
Multiple intensified performance of an enzyme-catalyzed reaction in organic medium.
作者信息
Yamane T, Iwasaki Y, Roxana R, Shimidzu N, Doisaki N
机构信息
Laboratory of Molecular Biotechnology, Nagoya University, Japan.
出版信息
Ann N Y Acad Sci. 1998 Dec 13;864:171-9. doi: 10.1111/j.1749-6632.1998.tb10299.x.
A lipase-catalyzed glycerolysis reaction (a transesterification between polyunsaturated fatty acid ethyl ester [PUFA] and glycerol) was investigated. Its performance was multiply intensified by (1) using a lipase having high specific activity, high activity in organic solvent, and high tolerance in organic solvent; (2) immobilization on fine CaCO3 powder (cheap and safe material, easy physical adsorption method of immobilization, reusable); (3) reaction in vacuo resulting in 100% conversion and effective avoidance of oxidative deterioration of PUFA; (4) high volumetric productivity because of no use of solvent; and (5) no need of further separation and purification of the oil product. It is emphasized that performance of biocatalytic reactions in organic media should be enhanced manifold for industrial implementation.
研究了脂肪酶催化的甘油解反应（多不饱和脂肪酸乙酯[PUFA]与甘油之间的酯交换反应）。通过以下方式可使其性能得到多重强化：(1) 使用具有高比活性、在有机溶剂中具有高活性以及对有机溶剂具有高耐受性的脂肪酶；(2) 固定在细碳酸钙粉末上（廉价且安全的材料，易于通过物理吸附方法固定，可重复使用）；(3) 在真空中进行反应，实现100%转化并有效避免PUFA的氧化变质；(4) 由于不使用溶剂，具有高体积产率；(5) 无需对油产品进行进一步的分离和纯化。需要强调的是，为了工业应用，有机介质中生物催化反应的性能应得到大幅提高。
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