Hu K, Yao X S, Dong A J, Kobayashi H, Iwasaki S, Jing Y K
Department of Natural Products Chemistry, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, People's Republic of China.
J Nat Prod. 1999 Feb;62(2):299-301. doi: 10.1021/np980101z.
During further bioactivity-guided fractionation, a new pregnane glycoside, hypoglaucin G (1), and a known compound, pregna-5, 16-dien-3beta-ol-20-one 3-O-alpha-L-rhamnopyranosyl-(1-->2)- [alpha-L-rhamnopyranosyl-(1-->4)] -beta-D-glucopyranoside (2), were isolated from the EtOH extract of Dioscorea collettii var. hypoglauca rhizomes, which induced morphological deformation of Pyricularia oryzae mycelia with minimum morphological deformation concentration values of 135 microM and 236 microM, respectively. The structure of 1 was established as 16beta-(4'-methyl-5'-O-beta-D-glucopyranosyl- pentanoxyl)-pre gn-5-en-3 beta-ol-20-one 3-O-alpha-L- rhamnopyranosyl-(1-->2)-[alpha-L-rhamnopyranosyl-(1-->4)]-beta-D- glucopyranoside on the basis of chemical evidence and spectral analysis, especially by 2D NMR techniques.
在进一步的生物活性导向分离过程中，从粉背薯蓣根茎的乙醇提取物中分离出一种新的孕甾烷糖苷，hypoglaucin G（1），以及一种已知化合物，孕甾-5,16-二烯-3β-醇-20-酮3-O-α-L-鼠李吡喃糖基-(1→2)-[α-L-鼠李吡喃糖基-(1→4)]-β-D-葡萄糖吡喃糖苷（2），它们分别以135μM和236μM的最小形态变形浓度值诱导稻瘟病菌丝体的形态变形。根据化学证据和光谱分析，特别是二维核磁共振技术，确定1的结构为16β-(4'-甲基-5'-O-β-D-葡萄糖吡喃糖基-戊酰氧基)-孕甾-5-烯-3β-醇-20-酮3-O-α-L-鼠李吡喃糖基-(1→2)-[α-L-鼠李吡喃糖基-(1→4)]-β-D-葡萄糖吡喃糖苷。
