香蕉束顶病毒DNA - 2至6是单顺反子的。

Banana bunchy top virus DNA-2 to 6 are monocistronic.

作者信息

Beetham P R, Harding R M, Dale J L

机构信息

Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, Brisbane, Australia.

出版信息

Arch Virol. 1999;144(1):89-105. doi: 10.1007/s007050050487.

DOI:10.1007/s007050050487

PMID:10076511

Abstract

Banana bunchy top virus (BBTV) DNA-3 to 6 have each previously been shown to contain one large open reading frame in the virion sense, whereas no large ORF had been identified in BBTV DNA-2. RNAs transcribed from the BBTV genome were mapped using northern hybridisation and 3' RACE. One mRNA was transcribed from each of BBTV DNA-2 to 6 and four of these mRNAs mapped to the ORFs previously identified in BBTV DNA-3 to 6. The mRNA of BBTV DNA-2 was transcribed from a virion sense ORF probably using a TATA box sequence different to that in BBTV DNA-1, and DNA-3 to 6. This ORF encoded a 10 kDa protein of unknown function. The 3' untranslated region of the five mRNAs varied from 25 nucleotides (BBTV DNA-6) to 167 nucleotides (BBTV DNA-4) and each contained putative polyadenylation signals with associated GT rich sequence together with a possible termination signal (C/T/A)TGTAA conserved in all five mRNAs.

摘要

香蕉束顶病毒（BBTV）的DNA - 3至6先前已显示在病毒粒子意义上各自含有一个大的开放阅读框，而在BBTV DNA - 2中未鉴定到大型开放阅读框。使用Northern杂交和3' RACE对从BBTV基因组转录的RNA进行定位。从BBTV DNA - 2至6各自转录出一种mRNA，其中四种mRNA与先前在BBTV DNA - 3至6中鉴定的开放阅读框相对应。BBTV DNA - 2的mRNA可能使用与BBTV DNA - 1以及DNA - 3至6中不同的TATA盒序列，从病毒粒子意义上的开放阅读框转录而来。该开放阅读框编码一种功能未知的10 kDa蛋白质。这五种mRNA的3'非翻译区从25个核苷酸（BBTV DNA - 6）到167个核苷酸（BBTV DNA - 4）不等，并且每种都包含推定的多聚腺苷酸化信号以及相关的富含GT的序列，同时还有一个可能的终止信号（C/T/A）TGTAA，在所有五种mRNA中均保守。