Katul L, Maiss E, Morozov S Y, Vetten H J
Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Braunschweig, Germany.
Virology. 1997 Jul 7;233(2):247-59. doi: 10.1006/viro.1997.8611.
Faba bean necrotic yellows virus (FBNYV) has a multicomponent circular ssDNA genome. In addition to a previously described genome component (C1) coding for a replicase-associated protein (Rep), five further components (C2 to C6) have now been identified. Each of the six components is about 1 kb in size, contains one major open reading frame (ORF) in the virion sense with a TATA box and polyadenylation signal, and has a noncoding region containing a highly conserved sequence possibly forming a stem-loop structure. Similar to C1, C2 encodes another putative Rep of 33.1 kDa, which is closely related to the Rep of banana bunchy top virus (BBTV). Based on bacterial expression and immunoblot analysis, the ORF of C5 encodes the capsid protein (CP) with a deduced molecular mass of 19 kDa. The FBNYV CP shares the highest amino acid (aa) identity (56.2%) with that of subterranean clover stunt virus (SCSV). The ORF of C4 potentially codes for a hydrophobic protein which appears to be structurally and functionally similar to the BBTV-C4 and SCSV-C1 proteins. No protein sequence similarities were found in databases for the C3 and C6 ORFs of FBNYV. FBNYV is clearly distinct from any known virus but is taxonomically related to BBTV and SCSV.
蚕豆坏死黄化病毒（FBNYV）具有多组分环状单链DNA基因组。除了先前描述的编码复制酶相关蛋白（Rep）的基因组组分（C1）外，现已鉴定出另外五个组分（C2至C6）。六个组分中的每一个大小约为1 kb，在病毒链意义上包含一个主要开放阅读框（ORF），带有TATA框和多聚腺苷酸化信号，并且具有一个非编码区，该非编码区包含可能形成茎环结构的高度保守序列。与C1相似，C2编码另一种推定的33.1 kDa Rep，它与香蕉束顶病毒（BBTV）的Rep密切相关。基于细菌表达和免疫印迹分析，C5的ORF编码衣壳蛋白（CP），推导的分子量为19 kDa。FBNYV CP与地下三叶草矮化病毒（SCSV）的CP具有最高的氨基酸（aa）同一性（56.2%）。C4的ORF可能编码一种疏水蛋白，该蛋白在结构和功能上似乎与BBTV-C4和SCSV-C1蛋白相似。在数据库中未发现FBNYV的C3和C6 ORF的蛋白质序列相似性。FBNYV明显不同于任何已知病毒，但在分类学上与BBTV和SCSV相关。
