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阻抗微生物学:通过电容生长曲线对牛奶中的细菌含量进行定量分析。

Impedance microbiology: quantification of bacterial content in milk by means of capacitance growth curves.

作者信息

Felice C J, Madrid R E, Olivera J M, Rotger V I, Valentinuzzi M E

机构信息

Instituto de Bioingeniería, FACET-UNT, Tucumán, Argentina.

出版信息

J Microbiol Methods. 1999 Feb;35(1):37-42. doi: 10.1016/s0167-7012(98)00098-0.

Abstract

The impedancimetric method is a technique for the rapid evaluation of milk bacterial content and also of its subproducts. Several authors have made use of culture conductance changes during bacterial growth for quantitative and qualitative assessments of microbial growth. However, interface capacitance curves, Ci, have not been used. In this paper, we quantify bacteria in cow raw milk by following their growth as the above-mentioned capacitance change time course event. With it, bigger growth variations, shorter detection times and a better coefficient of correlation with the plate count method were obtained than those yielded by conductance curves. Calibration was performed by plotting initial known concentrations, IC (CFU/ml), as a function of the time detection theshold (TDT).

摘要

阻抗测定法是一种用于快速评估牛奶细菌含量及其副产品的技术。几位作者利用细菌生长过程中的培养物电导变化对微生物生长进行定量和定性评估。然而,尚未使用界面电容曲线Ci。在本文中,我们通过跟踪上述电容变化时间过程事件中细菌的生长来对生鲜牛奶中的细菌进行定量。由此获得的生长变化更大、检测时间更短,并且与平板计数法的相关系数比电导曲线得到的更好。通过将初始已知浓度IC(CFU/ml)作为时间检测阈值(TDT)的函数进行绘图来进行校准。

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