Heegaard N H
Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark.
J Mol Recognit. 1998 Winter;11(1-6):141-8. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<141::AID-JMR410>3.0.CO;2-D.
Molecular recognition may be characterized both qualitatively and quantitatively by electrophoretic methods if complexed molecules differ in electrophoretic mobility from unbound ones. The use of capillary zone electrophoresis (CE) for the characterization of affinity interactions is advantageous because of the high resolution, reproducibility and wide applicability of the technique and because of the mild conditions, i.e., physiological buffers without additions of organics or detergents, that are often sufficient for highly efficient separations. CE gives the ability to characterize binding between small amounts of unlabelled reactants in solution, has few requirements for special characteristics of the interacting molecules and is also applicable to the study of interactions of individual components in mixtures, as detection of binding and analytical separation are achieved in one step. This is unique compared with other techniques for the study of non-covalent interactions. The advantages and disadvantages of using CE to demonstrate molecular interactions, to screen for specific ligand binding in complex mixtures and to calculate binding constants will be discussed.
如果复合分子的电泳迁移率与未结合分子不同，那么分子识别可以通过电泳方法进行定性和定量表征。使用毛细管区带电泳（CE）来表征亲和相互作用具有优势，这是因为该技术具有高分辨率、可重复性和广泛的适用性，还因为其条件温和，即使用生理缓冲液，无需添加有机物或去污剂，这些条件通常足以实现高效分离。CE能够表征溶液中少量未标记反应物之间的结合，对相互作用分子的特殊特性要求很少，并且还适用于研究混合物中单个组分的相互作用，因为结合检测和分析分离在一步中即可完成。与其他研究非共价相互作用的技术相比，这是独一无二的。将讨论使用CE来证明分子相互作用、筛选复杂混合物中的特异性配体结合以及计算结合常数的优缺点。
