Hage D S
Chemistry Department, University of Nebraska, Lincoln 68588-0304, USA.
Electrophoresis. 1997 Nov;18(12-13):2311-21. doi: 10.1002/elps.1150181222.
Proteins are receiving increased attention in capillary electrophoresis (CE) for use as ligands in chiral separations. This review examines the types of proteins that have been employed in CE for the analysis of chiral compounds and provides a summary of applications for such an approach. Several formats for this type of analysis are discussed, including methods that use immobilized proteins (i.e., affinity capillary electrochromatography and capillary gel affinity electrophoresis) or techniques that use proteins as running buffer additives (i.e., affinity capillary electrophoresis). The role of various experimental factors in these separations are considered, and a comparison is made between the general analytical properties of CE systems that use immobilized versus solution-phase proteins.
蛋白质作为手性分离中的配体，在毛细管电泳（CE）中受到越来越多的关注。本文综述了用于手性化合物分析的CE中所使用的蛋白质类型，并总结了这种方法的应用。讨论了这种分析类型的几种形式，包括使用固定化蛋白质的方法（即亲和毛细管电色谱和毛细管凝胶亲和电泳）或使用蛋白质作为运行缓冲液添加剂的技术（即亲和毛细管电泳）。考虑了各种实验因素在这些分离中的作用，并比较了使用固定化蛋白质与溶液相蛋白质的CE系统的一般分析特性。
