Ljungberg C, Novikov L, Kellerth J O, Ebendal T, Wiberg M
Department of Anatomy, Umeå University, and University Hospital, Sweden.
Neurosci Lett. 1999 Feb 26;262(1):29-32. doi: 10.1016/s0304-3940(99)00040-3.
The effect of three different neurotrophins on axotomy-induced death of adult rat sensory neurons was examined. The ventral branch of the 13th spinal nerve was transected and the corresponding neurons in the 13th thoracic (T13) dorsal root ganglion (DRG) were pre-labelled with Fast Blue (FB). For a period of 4 weeks, animals received either no treatment, continuous intrathecal infusion of phosphate buffer, nerve growth factor (NGF), neurotrophin-3 (NT-3), or brain-derived neurotrophic factor (BDNF). Labelled neurons remaining after this period were counted. Inert, or no treatment, resulted in extensive loss of the DRG neurons. BDNF application was virtually non-effective, while NGF or NT-3 resulted in a greater number of FB-labelled neurons compared to normal controls. This suggests that NGF and NT-3 are survival factors for adult sensory neurons with a therapeutic potential in peripheral nerve injuries.
研究了三种不同神经营养因子对成年大鼠感觉神经元轴突切断诱导死亡的影响。切断第13对脊神经的腹支，并用快蓝（FB）对第13胸段（T13）背根神经节（DRG）中的相应神经元进行预标记。在4周的时间里，动物分别接受无治疗、持续鞘内注射磷酸盐缓冲液、神经生长因子（NGF）、神经营养因子-3（NT-3）或脑源性神经营养因子（BDNF）。在此期间后剩余的标记神经元进行计数。无活性或不治疗导致DRG神经元大量丢失。应用BDNF几乎无效，而与正常对照组相比，NGF或NT-3导致更多的FB标记神经元。这表明NGF和NT-3是成年感觉神经元的存活因子，在外周神经损伤中具有治疗潜力。
