Tsatsakis A M, Psillakis T, Stefis A, Assithianakis P, Vlahonikolis I G, Michalodimitrakis M N, Helidonis E
Laboratory of Toxicology, Medical School, University of Crete, Iraklion, Voutes, Greece.
Boll Chim Farm. 1998 Dec;137(11):459-66.
Phenytoin (PH) levels were determined in the head hair of twenty five patients (fourteen males and eleven females, aged from five to seventy seven years old) who were receiving this drug chronically. The assay method involved sectional hair analysis by dissolution and liquid phase extraction procedures, using both high pressure liquid chromatography (HPLC) and immunoassay (Abbott TDx) techniques. Five sections were studied, the first being the one closet to the root. The phenytoin levels of the hair sections ranged: a) 1st: 0.68 to 39.56 micrograms/g (mean 16.12 micrograms/g), b) 2nd: 0.85 to 34.15 micrograms/g (mean 9.45 micrograms/g), c) 3rd: 1.33 to 27.90 micrograms/g (mean 3.72 micrograms/g), d) 4th: 1.10 to 10.96 micrograms/g (mean 3.04 micrograms/g) and e) 5th: 1.05 to 7.00 micrograms/g (mean 2.84 micrograms/g) according to the HPLC analysis. The immunoassay technique gave similar results. The mean values of phenytoin in the hair sections according to the immunoassay technique were: 16.28; 9.47; 3.77; 3.22; 2.97 micrograms/g, respectively from the 1st to the 5th section. A reduction of drug concentrations in hair from the first to the consecutive segments was observed. Higher amounts of phenytoin were deposited in black, untreated hair in comparison to blond brown or grey hair. Phenytoin concentrations in hair sections correlated with the oral daily dosage of the drug. Our data indicate to the use of hair testing as a marker of the dosage history and evaluation of the compliance of patients under long treatment with phenytoin.
对25例长期服用苯妥英钠的患者（14名男性和11名女性，年龄5至77岁）的头发进行了苯妥英钠（PH）水平测定。检测方法包括通过溶解和液相萃取程序进行分段头发分析，同时使用高压液相色谱（HPLC）和免疫测定（雅培TDx）技术。研究了五个部分，第一部分是最靠近发根的部分。根据HPLC分析，各段头发中苯妥英钠水平范围为：a）第一段：0.68至39.56微克/克（平均16.12微克/克），b）第二段：0.85至34.15微克/克（平均9.45微克/克），c）第三段：1.33至27.90微克/克（平均3.72微克/克），d）第四段：1.10至10.96微克/克（平均3.04微克/克），e）第五段：1.05至7.00微克/克（平均2.84微克/克）。免疫测定技术给出了类似结果。根据免疫测定技术，各段头发中苯妥英钠的平均值分别为：第一段至第五段分别为16.28；9.47；3.77；3.22；2.97微克/克。观察到从第一段到连续段头发中药物浓度降低。与金色、棕色或灰色头发相比，黑色未处理头发中沉积的苯妥英钠含量更高。头发段中的苯妥英钠浓度与药物的口服日剂量相关。我们的数据表明，头发检测可作为剂量历史的标志物以及评估长期接受苯妥英钠治疗患者的依从性。
